Evidence map›Paper›PMID 16228393›Full record

ArticlePhotosynthesis research2004

Mass Spectrometric Evidence for an Alternate Disulfide Bond in Chloroplast Fructose Bisphosphatase.

Wei Wu, J Throck Watson, Fred J Stevens, Ryan Yousefzai, Louise E Anderson

Abstract read
PubMed Publisher
In one paragraph

Article in Photosynthesis research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Wei WuDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
J Throck WatsonDepartment of Chemistry, Michigan State University, East Lansing, MI, 48824, USA.
Fred J StevensBiosciences Division, Argonne National Laboratory, Argonne, IL, 60439, USA.
Ryan YousefzaiDepartment of Biological Sciences, University of Illinois-Chicago, 845 West Taylor, Chicago, IL, 60680, USA.
Louise E AndersonDepartment of Biological Sciences, University of Illinois-Chicago, 845 West Taylor, Chicago, IL, 60680, USA.
Michigan State University · USUniversity of Illinois Chicago · USArgonne National Laboratory · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mass mapping analysis based on cyanylation (CN) of the protein and CN-induced cleavage indicates that all three cysteine residues in the insertion into the light-activated pea leaf chloroplast fructose bisphosphatase (E.C. 3.1.3.11) are able to participate in disulfide bond formation. There is a major peak in the mass spectrum of the cleavage products indicating that Cys173 forms a disulfide bond with Cys153, consistent with the structure of the oxidized enzyme in PDB files 1d9q and 1dcu, and a minor peak indicating that Cys173 forms an alternate disulfide bond with Cys178. The Cys173-Cys178 disulfide bond was not apparent in the available crystal structures.

Indexed as

alternate disulfide bondsCN-induced cleavagefructose bisphosphataselight-activationmass mappingredox-regulation

Identifiers

PMID16228393
OpenAlexW2016424131

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.