ArticleMolecular and cellular biology2005
PTB regulates the processing of a 3'-terminal exon by repressing both splicing and polyadenylation.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- CRISPR/Cas9 screening with destabilized bicistronic fluorescent protein reporter revealed PABPN1 as a hub of regulators for alternative polyadenylation.Nucleic acids research · 2026Article
- Regulation of alternative splicing and polyadenylation in neurons.Life science alliance · 2023Review
- Activation and inhibition of nonsense-mediated mRNA decay control the abundance of alternative polyadenylation products.Nucleic acids research · 2020Article
- CD5 expression is regulated during human T-cell activation by alternative polyadenylation, PTBP1, and miR-204.European journal of immunology · 2016Article
- Polarizing the Neuron through Sustained Co-expression of Alternatively Spliced Isoforms.Cell reports · 2016Article
- A posttranscriptional mechanism that controls Ptbp1 abundance in the Xenopus epidermis.Molecular and cellular biology · 2015Article
- Article
- Implication of the SMN complex in the biogenesis and steady state level of the signal recognition particle.Nucleic acids research · 2013Article
- Characterization of the distal polyadenylation site of the ß-adducin (Add2) pre-mRNA.PloS one · 2013Article
- Loss of PTB or negative regulation of Notch mRNA reveals distinct zones of Notch and actin protein accumulation in Drosophila embryo.PloS one · 2011Article
- HuB/C/D, nPTB, REST4, and miR-124 regulators of neuronal cell identity are also utilized in the lens.Molecular vision · 2010Article
- A potential regulatory role for mRNA secondary structures within the prothrombin 3'UTR.Thrombosis research · 2010Article
- Molecular mechanisms of eukaryotic pre-mRNA 3' end processing regulation.Nucleic acids research · 2010Review
- Next-generation SELEX identifies sequence and structural determinants of splicing factor binding in human pre-mRNA sequence.RNA (New York, N.Y.) · 2009Article
- Polypyrimidine tract binding protein prevents activity of an intronic regulatory element that promotes usage of a composite 3'-terminal exon.The Journal of biological chemistry · 2009Article
- Analysis of splicing patterns by pyrosequencing.Nucleic acids research · 2009Article
- Multiple roles for polypyrimidine tract binding (PTB) proteins in trypanosome RNA metabolism.RNA (New York, N.Y.) · 2009Article
- hnRNP I inhibits Notch signaling and regulates intestinal epithelial homeostasis in the zebrafish.PLoS genetics · 2009Article
- Polypyrimidine tract binding protein induces human papillomavirus type 16 late gene expression by interfering with splicing inhibitory elements at the major late 5' splice site, SD3632.Journal of virology · 2008Article
- The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit.Molecular and cellular biology · 2006Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polypyrimidine tract binding protein (PTB) has been described as a global repressor of regulated exons. To investigate PTB functions in a physiological context, we used a combination of morpholino-mediated knockdown and transgenic overexpression strategies in Xenopus laevis embryos. We show that embryonic endoderm and skin deficient in PTB displayed a switch of the alpha-tropomyosin pre-mRNA 3' end processing to the somite-specific pattern that results from the utilization of an upstream 3'-terminal exon designed exon 9A9'. Conversely, somitic targeted overexpression of PTB resulted in the repression of the somite-specific exon 9A9' and a switch towards the nonmuscle pattern. These results validate PTB as a key physiological regulator of the 3' end processing of the alpha-tropomyosin pre-mRNA. Moreover, using a minigene strategy in the Xenopus oocyte, we show that in addition to repressing the splicing of exon 9A9', PTB regulates the cleavage/polyadenylation of this 3'-terminal exon.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.