ArticleMolecular and cellular biology2005
Systematic study of sequence motifs for RNA trans splicing in Trypanosoma brucei.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 78 citations in OpenAlex.
- Structural basis of step II spliced leader RNA trans-splicing in trypanosomatid parasites.Nature communications · 2026Article
- Post-transcriptional reprogramming by thousands of mRNA untranslated regions in trypanosomes.Nature communications · 2024Article
- Article
- Sequences and proteins that influence mRNA processing in Trypanosoma brucei: Evolutionary conservation of SR-domain and PTB protein functions.PLoS neglected tropical diseases · 2022Article
- TheRNA (New York, N.Y.) · 2022Article
- Article
- Upstream ORFs Influence Translation Efficiency in the ParasiteFrontiers in genetics · 2020Article
- Regulation of gene expression in trypanosomatids: living with polycistronic transcription.Open biology · 2019Article
- Exploiting CRISPR-Cas9 technology to investigate individual histone modifications.Nucleic acids research · 2018Article
- Article
- Landscape of the spliced leader trans-splicing mechanism in Schistosoma mansoni.Scientific reports · 2018Article
- UTRme: A Scoring-Based Tool to Annotate Untranslated Regions in Trypanosomatid Genomes.Frontiers in genetics · 2018Article
- Article
- Variant surface glycoprotein density defines an immune evasion threshold for African trypanosomes undergoing antigenic variation.Nature communications · 2017Article
- GT-rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes.The EMBO journal · 2017Article
- Article
- Integrating ribosomal promoter vectors that offer a choice of constitutive expression profiles in Leishmania donovani.Molecular and biochemical parasitology · 2015Article
- Transcriptomic profiling of gene expression and RNA processing during Leishmania major differentiation.Nucleic acids research · 2015Article
- The selenocysteine tRNA gene in leishmania major is transcribed by both RNA polymerase II and RNA polymerase III.Eukaryotic cell · 2015Article
- Transcriptome-wide analysis of trypanosome mRNA decay reveals complex degradation kinetics and suggests a role for co-transcriptional degradation in determining mRNA levels.Molecular microbiology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
mRNA maturation in Trypanosoma brucei depends upon trans splicing, and variations in trans-splicing efficiency could be an important step in controlling the levels of individual mRNAs. RNA splicing requires specific sequence elements, including conserved 5' splice sites, branch points, pyrimidine-rich regions [poly(Y) tracts], 3' splice sites (3'SS), and sometimes enhancer elements. To analyze sequence requirements for efficient trans splicing in the poly(Y) tract and around the 3'SS, we constructed a luciferase-beta-galactosidase double-reporter system. By testing approximately 90 sequences, we demonstrated that the optimum poly(Y) tract length is approximately 25 nucleotides. Interspersing a purely uridine-containing poly(Y) tract with cytidine resulted in increased trans-splicing efficiency, whereas purines led to a large decrease. The position of the poly(Y) tract relative to the 3'SS is important, and an AC dinucleotide at positions -3 and -4 can lead to a 20-fold decrease in trans splicing. However, efficient trans splicing can be restored by inserting a second AG dinucleotide downstream, which does not function as a splice site but may aid in recruitment of the splicing machinery. These findings should assist in the development of improved algorithms for computationally identifying a 3'SS and help to discriminate noncoding open reading frames from true genes in current efforts to annotate the T. brucei genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.