ArticleBiophysical journal2006
Functional Na+ channels in cell adhesion probed by transistor recording.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Two firing modes and well-resolved NaPflugers Archiv : European journal of physiology · 2023Article
- Plasticity and Adaptation in Neuromorphic Biohybrid Systems.iScience · 2020Review
- Current-Induced Transistor Sensorics with Electrogenic Cells.Biosensors · 2016Review
- Electrical interfacing of neurotransmitter receptor and field effect transistor.The European physical journal. E, Soft matter · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell membranes in a tissue are in close contact to each other, embedded in the extracellular matrix. Standard electrophysiological methods are not able to characterize ion channels under these conditions. Here we consider the area of cell adhesion on a solid substrate as a model system. We used HEK 293 cells cultured on fibronectin and studied the activation of Na(V)1.4 sodium channels in the adherent membrane with field-effect transistors in a silicon substrate. Under voltage clamp, we compared the transistor response with the whole-cell current. We observed that the extracellular voltage in the cell-chip contact was proportional to the total membrane current. The relation was calibrated by alternating-current stimulation. We found that Na(+) channels are present in the area of cell adhesion on fibronectin with a functionality and a density that is indistinguishable from the free membrane. The experiment provides a basis for studying selective accumulation and depletion of ion channels in cell adhesion and also for a development of cell-based biosensoric devices and neuroelectronic systems.
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Registered trials
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