ArticleBiophysical journal2006
Fluorescence measurement of intracellular sodium concentration in single Escherichia coli cells.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- Toolbox of FRET-based c-di-GMP biosensors and its FRET-To-Sort application for genome-wide mapping of c-di-GMP regulation.Nature communications · 2026Article
- Elevator mechanism dynamics in a sodium-coupled dicarboxylate transporter.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Evidence for ecological adaptation of reductive citric acid and reverse oxidative citric acid cycles based on a survey of genomes from autotrophicFEMS microbes · 2026Review
- QuantitativeMagma (New York, N.Y.) · 2024Article
- The accumulation and growth of Pseudomonas aeruginosa on surfaces is modulated by surface mechanics via cyclic-di-GMP signaling.NPJ biofilms and microbiomes · 2023Article
- Measurements of the Ion Channel Activity of the Transmembrane Stator Complex in the Bacterial Flagellar Motor.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- The Dynamic Ion Motive Force Powering the Bacterial Flagellar Motor.Frontiers in microbiology · 2021Review
- Stator Dynamics Depending on Sodium Concentration in Sodium-Driven Bacterial Flagellar Motors.Frontiers in microbiology · 2021Article
- Diverse Physiological Functions of Cation Proton Antiporters across Bacteria and Plant Cells.International journal of molecular sciences · 2020Review
- Article
- Bacterial Intracellular Sodium Ion Measurement using CoroNa Green.Bio-protocol · 2017Article
- The Bacterial Flagellar Type III Export Gate Complex Is a Dual Fuel Engine That Can Use Both H+ and Na+ for Flagellar Protein Export.PLoS pathogens · 2016Article
- Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF.Scientific reports · 2015Article
- Minimization of extracellular space as a driving force in prokaryote association and the origin of eukaryotes.Biology direct · 2014Article
- Hybrid-fuel bacterial flagellar motors in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Temperature dependences of torque generation and membrane voltage in the bacterial flagellar motor.Biophysical journal · 2013Article
- Mechanism and kinetics of a sodium-driven bacterial flagellar motor.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Inferring diffusion in single live cells at the single-molecule level.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Solute Carrier Family SLC41, what do we really know about it?Wiley interdisciplinary reviews. Membrane transport and signaling · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The energy-transducing cytoplasmic membrane of bacteria contains pumps and antiports maintaining the membrane potential and ion gradients. We have developed a method for rapid, single-cell measurement of the internal sodium concentration ([Na(+)](in)) in Escherichia coli using the sodium ion fluorescence indicator, Sodium Green. The bacterial flagellar motor is a molecular machine that couples the transmembrane flow of ions, either protons (H(+)) or sodium ions (Na(+)), to flagellar rotation. We used an E. coli strain containing a chimeric flagellar motor with H(+)- and Na(+)-driven components that functions as a sodium motor. Changing external sodium concentration ([Na(+)](ex)) in the range 1-85 mM resulted in changes in [Na(+)](in) between 5-14 mM, indicating a partial homeostasis of internal sodium concentration. There were significant intercell variations in the relationship between [Na(+)](in) and [Na(+)](ex), and the internal sodium concentration in cells not expressing chimeric flagellar motors was 2-3 times lower, indicating that the sodium flux through these motors is a significant fraction of the total sodium flux into the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.