ArticleProceedings of the National Academy of Sciences of the United States of America2005
Functions and dysfunctions of the nuclear lamin Ig-fold domain in nuclear assembly, growth, and Emery-Dreifuss muscular dystrophy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 50 citations in OpenAlex.
- Nuclear cholesterol regulates nuclear size and DNA damage responses in cancer stem cells.Neuro-oncology · 2025Article
- The lamin A/C Ig-fold undergoes cell density-dependent changes that alter epitope binding.Nucleus (Austin, Tex.) · 2023Article
- The nuclear lamina couples mechanical forces to cell fate in the preimplantation embryo via actin organization.Nature communications · 2023Article
- Nde1 is required for heterochromatin compaction and stability in neocortical neurons.iScience · 2022Article
- The plant nuclear lamina proteins NMCP1 and NMCP2 form a filamentous network with lateral filament associations.Journal of experimental botany · 2021Article
- Article
- A nuclear lamina-chromatin-Ran GTPase axis modulates nuclear import and DNA damage signaling.Aging cell · 2019Article
- Non-equivalence of nuclear import among nuclei in multinucleated skeletal muscle cells.Journal of cell science · 2018Article
- The structure of lamin filaments in somatic cells as revealed by cryo-electron tomography.Nucleus (Austin, Tex.) · 2017Review
- Review
- Recent advances in understanding nuclear size and shape.Nucleus (Austin, Tex.) · 2016Review
- Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells.The Journal of biological chemistry · 2015Article
- Altering lamina assembly reveals lamina-dependent and -independent functions for A-type lamins.Journal of cell science · 2015Article
- Ameliorating pathogenesis by removing an exon containing a missense mutation: a potential exon-skipping therapy for laminopathies.Gene therapy · 2015Article
- Sizing and shaping the nucleus: mechanisms and significance.Current opinion in cell biology · 2014Review
- Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation.Science (New York, N.Y.) · 2013Article
- From lamins to lamina: a structural perspective.Histochemistry and cell biology · 2013Review
- Structure and stability of the lamin A tail domain and HGPS mutant.Journal of structural biology · 2011Article
- Article
- Nuclear lamins.Cold Spring Harbor perspectives in biology · 2010Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
The non-alpha-helical C terminus of Xenopus lamin B3 (LB3T) inhibits the polymerization of lamin B3 in vitro and prevents the assembly of nuclei in Xenopus egg interphase extracts. To more precisely define the functions of LB3T in nuclear assembly, we have expressed subdomains of LB3T and determined their effects on nuclear assembly in Xenopus extracts. The results demonstrate that the Ig-fold motif (LB3T-Ig) is sufficient to inhibit lamin polymerization in vitro. Addition of the LB3T-Ig to egg extracts before the introduction of chromatin prevents chromatin decondensation and the assembly of the lamina, membranes, and pore complexes comprising the nuclear envelope. When added to assembled nuclei, LB3T-Ig prevents the further incorporation of lamin B3 into the endogenous lamina and blocks nuclear growth. The introduction of a point mutation in LB3T-Ig (R454W; LB3T-IgRW), known to cause Emery-Dreifuss muscular dystrophy when present in lamin A, does not inhibit lamin polymerization, chromatin decondensation, or nuclear assembly and growth. These results shed light on the specific alterations in lamin functions attributable to a known muscular dystrophy mutation and provide an experimental framework for revealing the effects of other mutations causing a wide range of laminopathies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.