ArticleJournal of virology2005
S-phase-dependent enhancement of dengue virus 2 replication in mosquito cells, but not in human cells.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- Multiple functions of the nonstructural protein 3D in picornavirus infection.Frontiers in immunology · 2024Review
- Pathway analysis of host responses to dengue virus serotype 2 infection and inhibition of viral envelope protein by naringenin fromJournal of biosciences · 2023Article
- Life as a Vector of Dengue Virus: The Antioxidant Strategy of Mosquito Cells to Survive Viral Infection.Antioxidants (Basel, Switzerland) · 2021Review
- PRRSV Promotes MARC-145 Cells Entry Into S Phase of the Cell Cycle to Facilitate Viral ReplicationFrontiers in veterinary science · 2021Article
- Intra-host growth kinetics of dengue virus in the mosquito Aedes aegypti.PLoS pathogens · 2019Article
- A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti.PLoS neglected tropical diseases · 2018Article
- Article
- Coxsackievirus A6 Induces Cell Cycle Arrest in G0/G1 Phase for Viral Production.Frontiers in cellular and infection microbiology · 2018Article
- Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment.Acta pharmacologica Sinica · 2017Article
- Meta-Analysis ofFrontiers in bioengineering and biotechnology · 2017Article
- Human Enterovirus 68 Interferes with the Host Cell Cycle to Facilitate Viral Production.Frontiers in cellular and infection microbiology · 2017Article
- Iminosugars Inhibit Dengue Virus Production via Inhibition of ER Alpha-Glucosidases--Not Glycolipid Processing Enzymes.PLoS neglected tropical diseases · 2016Article
- Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D.Cell cycle (Georgetown, Tex.) · 2015Article
- High-throughput quantitative proteomic analysis of dengue virus type 2 infected A549 cells.PloS one · 2014Article
- Article
- Flaviviruses are sensitive to inhibition of thymidine synthesis pathways.Journal of virology · 2013Article
- Differential RNA sequence requirement for dengue virus replication in mosquito and mammalian cells.Journal of virology · 2013Article
- Article
- Global cross-talk of genes of the mosquito Aedes aegypti in response to dengue virus infection.PLoS neglected tropical diseases · 2011Article
- A single amino acid in nonstructural protein NS4B confers virulence to dengue virus in AG129 mice through enhancement of viral RNA synthesis.Journal of virology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Dengue virus (DEN) is the most prevalent cause of arthropod-borne viral illness in humans. We determined the influence of cellular growth state on DEN type 2 (DEN2) replication in mosquito and human cells, based on the hypothesis that manipulation of cellular growth state will facilitate identification of viral and cellular determinants of productive infection. Comparison of density-arrested and cycling C6/36 Aedes albopictus cells infected with a low-passage DEN2 isolate revealed that cycling cells generated higher virus titers per cell. When C6/36 cells were stalled in S-phase via a thymidine (THY) block, titers of low-passage DEN2 isolates and a high-passage strain, 16681, were increased approximately 30-fold and 10-fold, respectively. Moreover, virus release was earlier in THY-treated cells than in asynchronously cycling cells. Adsorption, entry, genome uncoating, and translation were not responsible for increased titers of virus from S-phase C6/36 cells. In contrast to the 30-fold increase in virus titers, intracellular levels of viral RNA were increased approximately 2-fold, suggesting that the S-phase-responsive step is late in the DEN2 replication cycle. Analysis of viral RNA and protein released from the cells indicated that enhanced DEN2 assembly is largely responsible for increased virus titers produced during S-phase. In contrast to C6/36 cells, DEN2 titers from S-phase human hepatoma cells or primary human fibroblasts were not increased. These results demonstrate a differential response of DEN2 to the mosquito and human cell cycle and provide a framework for detailed studies into the mechanisms mediating virus assembly.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.