ArticleBMC genomics2005
Macrodissection versus microdissection of rectal carcinoma: minor influence of stroma cells to tumor cell gene expression profiles.
Article in BMC genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Combined use of laser capture microdissection and cDNA microarray analysis identifies locally expressed disease-related genes in focal regions of psoriasis vulgaris skin lesions.The Journal of investigative dermatology · 2012Trial
- Human prostate organoid generation and the identification of prostate development drivers using inductive rodent tissues.Development (Cambridge, England) · 2023Article
- Artificial intelligence-augmented histopathologic review using image analysis to optimize DNA yield from formalin-fixed paraffin-embedded slides.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2022Article
- Impact of thymidine phosphorylase and CD163 expression on prognosis in stage II colorectal cancer.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2022Article
- Enhancing Tumor Content through Tumor Macrodissection.Journal of visualized experiments : JoVE · 2022Article
- Development of a predictive model for stromal content in prostate cancer samples to improve signature performance.The Journal of pathology · 2019Article
- Genome-wide analysis of androgen receptor binding and transcriptomic analysis in mesenchymal subsets during prostate development.Disease models & mechanisms · 2019Article
- The influence of cancer tissue sampling on the identification of cancer characteristics.Scientific reports · 2015Article
- Efficacy and feasibility of the epithelial cell adhesion molecule (EpCAM) immunomagnetic cell sorter for studies of DNA methylation in colorectal cancer.International journal of molecular sciences · 2013Article
- A mill based instrument and software system for dissecting slide-mounted tissue that provides digital guidance and documentation.BMC clinical pathology · 2013Article
- PDGFRB promotes liver metastasis formation of mesenchymal-like colorectal tumor cells.Neoplasia (New York, N.Y.) · 2013Article
- MS4A1 dysregulation in asbestos-related lung squamous cell carcinoma is due to CD20 stromal lymphocyte expression.PloS one · 2012Article
- Toll-like receptor 4 variant D299G induces features of neoplastic progression in Caco-2 intestinal cells and is associated with advanced human colon cancer.Gastroenterology · 2011Article
- Cross-species hybridization of microarrays for studying tumor transcriptome of brain metastasis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Distinctions in gastric cancer gene expression signatures derived from laser capture microdissection versus histologic macrodissection.BMC medical genomics · 2011Article
- Allelic variation at the 8q23.3 colorectal cancer risk locus functions as a cis-acting regulator of EIF3H.PLoS genetics · 2010Article
- The colorectal cancer risk at 18q21 is caused by a novel variant altering SMAD7 expression.Genome research · 2009Article
- Analysis of differential gene expression in colorectal cancer and stroma using fluorescence-activated cell sorting purification.British journal of cancer · 2009Article
- Impact of sample acquisition and linear amplification on gene expression profiling of lung adenocarcinoma: laser capture micro-dissection cell-sampling versus bulk tissue-sampling.BMC medical genomics · 2009Article
- Integrating chromosomal aberrations and gene expression profiles to dissect rectal tumorigenesis.BMC cancer · 2008Article
Corrections and comments
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Authors and funding
13 authors.
Funding
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Abstract
backgroundThe molecular determinants of carcinogenesis, tumor progression and patient prognosis can be deduced from simultaneous comparison of thousands of genes by microarray analysis. However, the presence of stroma cells in surgically excised carcinoma tissues might obscure the tumor cell-specific gene expression profiles of these samples. To circumvent this complication, laser microdissection can be performed to separate tumor epithelium from the surrounding stroma and healthy tissue. In this report, we compared RNAs isolated from macrodissected, of which only surrounding healthy tissue had been removed, and microdissected rectal carcinoma samples by microarray analysis in order to determine the most reliable approach to detect the expression of tumor cell-derived genes by microarray analysis.
resultsAs microdissection yielded low tissue and RNA quantities, extra rounds of mRNA amplification were necessary to obtain sufficient RNA for microarray experiments. These second rounds of amplification influenced the gene expression profiles. Moreover, the presence of stroma cells in macrodissected samples had a minor contribution to the tumor cell gene expression profiles, which can be explained by the observation that more RNA is extracted from tumor epithelial cells than from stroma.
conclusionThese data demonstrate that the more convenient procedure of macrodissection can be adequately used and yields reliable data regarding the identification of tumor cell-specific gene expression profiles.
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