ArticleNucleic acids research2005
A simple method for displaying recalcitrant proteins on the surface of bacteriophage lambda.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 36 citations in OpenAlex.
- A λ Phage Platform for Successful Therapeutic Display of Protein Antigens.Research square · 2026Article
- A λ Phage Platform for Successful Therapeutic Display of Protein Antigens.bioRxiv : the preprint server for biology · 2026Article
- Engineered bacteriophage-based bioimaging Technology: Development and applications.Synthetic and systems biotechnology · 2025Review
- Article
- A Lambda-evo (λApplied microbiology and biotechnology · 2025Article
- Bacteriophage T4 as a Protein-Based, Adjuvant- and Needle-Free, Mucosal Pandemic Vaccine Design Platform.Annual review of virology · 2024Review
- Article
- Plant/Bacterial Virus-Based Drug Discovery, Drug Delivery, and Therapeutics.Pharmaceutics · 2019Review
- Bacteriophage T4 nanoparticles for vaccine delivery against infectious diseases.Advanced drug delivery reviews · 2019Review
- Construction and analysis of a genetically tuneable lytic phage display system.Applied microbiology and biotechnology · 2013Article
- Article
- Decision making at a subcellular level determines the outcome of bacteriophage infection.Cell · 2010Article
- Progress in phage display: evolution of the technique and its application.Cellular and molecular life sciences : CMLS · 2010Review
- Proteasome inhibitors enhance bacteriophage lambda (lambda) mediated gene transfer in mammalian cells.Virology · 2009Article
- Fc receptor-mediated, antibody-dependent enhancement of bacteriophage lambda-mediated gene transfer in mammalian cells.Virology · 2008Article
- In vivo gene delivery and expression by bacteriophage lambda vectors.Journal of applied microbiology · 2007Article
- A tractable method for simultaneous modifications to the head and tail of bacteriophage lambda and its application to enhancing phage-mediated gene delivery.Nucleic acids research · 2007Article
- Display technologies: application for the discovery of drug and gene delivery agents.Advanced drug delivery reviews · 2006Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Bacteriophage lambda (lambda) permits the display of many foreign peptides and proteins on the gpD major coat protein. However, some recombinant derivatives of gpD are incompatible with the assembly of stable phage particles. This presents a limitation to current lambda display systems. Here we describe a novel, plasmid-based expression system in which gpD deficient lambda lysogens can be co-complemented with both wild-type and recombinant forms of gpD. This dual expression system permits the generation of mosaic phage particles that contain otherwise recalcitrant recombinant gpD fusion proteins. Overall, this improved gpD display system is expected to permit the expression of a wide variety of peptides and proteins on the surface of bacteriophage lambda and to facilitate the use of modified lambda phage vectors in mammalian gene transfer applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.