ArticleJournal of biomolecular NMR2005
Optimization of resolution and sensitivity of 4D NOESY using multi-dimensional decomposition.
Article in Journal of biomolecular NMR, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed.
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- Codon Harmonization of a Kir3.1-KirBac1.3 Chimera for Structural Study Optimization.Biomolecules · 2020Article
- Comparison of backbone dynamics of the p50 dimerization domain of NFκB in the homodimeric transcription factor NFκB1 and in its heterodimeric complex with RelA (p65).Protein science : a publication of the Protein Society · 2019Article
- Automated assignment of NMR chemical shifts based on a known structure and 4D spectra.Journal of biomolecular NMR · 2016Article
- HN-NCA heteronuclear TOCSY-NH experiment for (1)H(N) and (15)N sequential correlations in ((13)C, (15)N) labelled intrinsically disordered proteins.Journal of biomolecular NMR · 2015Article
- Non-Uniform Sampling and J-UNIO Automation for Efficient Protein NMR Structure Determination.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
- Development of a method for reconstruction of crowded NMR spectra from undersampled time-domain data.Journal of biomolecular NMR · 2015Article
- Practical aspects of NMR signal assignment in larger and challenging proteins.Progress in nuclear magnetic resonance spectroscopy · 2014Review
- Time-resolved multidimensional NMR with non-uniform sampling.Journal of biomolecular NMR · 2014Article
- Sampling scheme and compressed sensing applied to solid-state NMR spectroscopy.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2013Article
- NMR methods for structural studies of large monomeric and multimeric proteins.Current opinion in structural biology · 2013Review
- 4D Non-uniformly sampled C,C-NOESY experiment for sequential assignment of 13C, 15N-labeled RNAs.Journal of biomolecular NMR · 2013Article
- Selective diagonal-free (13)C, (13)C-edited aliphatic-aromatic NOESY experiment with non-uniform sampling.Journal of biomolecular NMR · 2013Article
- Article
- Deterministic schedules for robust and reproducible non-uniform sampling in multidimensional NMR.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2012Article
- Exclusively NOESY-based automated NMR assignment and structure determination of proteins.Journal of biomolecular NMR · 2011Article
- A novel strategy for NMR resonance assignment and protein structure determination.Journal of biomolecular NMR · 2011Article
- NleG Type 3 effectors from enterohaemorrhagic Escherichia coli are U-Box E3 ubiquitin ligases.PLoS pathogens · 2010Article
- Biomolecular NMR data analysis.Progress in nuclear magnetic resonance spectroscopy · 2010Review
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Highly resolved multi-dimensional NOE data are essential for rapid and accurate determination of spatial protein structures such as in structural genomics projects. Four-dimensional spectra contain almost no spectral overlap inherently present in lower dimensionality spectra and are highly amenable to application of automated routines for spectral resonance location and assignment. However, a high resolution 4D data set using conventional uniform sampling usually requires unacceptably long measurement time. Recently we have reported that the use of non-uniform sampling and multi-dimensional decomposition (MDD) can remedy this problem. Here we validate accuracy and robustness of the method, and demonstrate its usefulness for fully protonated protein samples. The method was applied to 11 kDa protein PA1123 from structural genomics pipeline. A systematic evaluation of spectral reconstructions obtained using 15-100% subsets of the complete reference 4D 1H-13C-13C-1H NOESY spectrum has been performed. With the experimental time saving of up to six times, the resolution and the sensitivity per unit time is shown to be similar to that of the fully recorded spectrum. For the 30% data subset we demonstrate that the intensities in the reconstructed and reference 4D spectra correspond with a correlation coefficient of 0.997 in the full range of spectral amplitudes. Intensities of the strong, middle and weak cross-peaks correlate with coefficients 0.9997, 0.9965, and 0.83. The method does not produce false peaks. 2% of weak peaks lost in the 30% reconstruction is in line with theoretically expected noise increase for the shorter measurement time. Together with good accuracy in the relative line-widths these translate to reliable distance constrains derived from sparsely sampled, high resolution 4D NOESY data.
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