ArticleNucleic acids research2005
Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Status quo of tet regulation in bacteria.Microbial biotechnology · 2022Review
- Improving the efficiency of transposon mutagenesis in Salmonella enteritidis by overcoming host-restriction barriers.Molecular biotechnology · 2014Article
- Development of a high-efficient transformation system of Bacillus pumilus strain DX01 to facilitate gene isolation via gfp-tagged insertional mutagenesis and visualize bacterial colonization of rice roots.Folia microbiologica · 2013Article
- An update on the molecular genetics toolbox for staphylococci.Microbiology (Reading, England) · 2013Review
- High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.BMC genomics · 2012Article
- Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis.Journal of bacteriology · 2011Article
- New architectures for Tet-on and Tet-off regulation in Staphylococcus aureus.Applied and environmental microbiology · 2010Article
- The application of Tet repressor in prokaryotic gene regulation and expression.Microbial biotechnology · 2008Review
- Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.Applied and environmental microbiology · 2006Article
- Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase.Applied and environmental microbiology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the construction and application of elements for random insertion of promoter containing DNA into the genome of Bacillus subtilis. The outward-facing promoter of these integrative elements termed InsTet(G+) is inducible by tetracycline so that conditional mutants are generated. We constructed three InsTet(G+) variants using different regulatory windows. In the first, the regulator gene tetR is located within the element, allowing one-step mutagenesis. The second contains tetR in the chromosome and yields the best regulation efficiency. The third exploits xylose-dependent tetR expression from a plasmid, enabling induction of TetR synthesis so that distinct expression levels of an affected gene can be adjusted. We have obtained mutant strains with all three variants. For some of them, growth can be modulated by the presence of effectors. Most growth defects occur in the presence of inducers, presumably due to regulated expression of antisense RNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.