ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Endocannabinoid signaling dynamics probed with optical tools.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 71 citations in OpenAlex.
- Distinct endocannabinoids specifically signal to astrocytes or neurons in the adult mouse hippocampus.Nature neuroscience · 2026Article
- Article
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Chemical Probes to Control and Visualize Lipid Metabolism in the Brain.Accounts of chemical research · 2022Review
- A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo.Nature biotechnology · 2022Article
- 2-Arachidonoylglycerol mobilization following brief synaptic stimulation in the dorsal lateral striatum requires glutamatergic and cholinergic neurotransmission.Neuropharmacology · 2022Article
- Imaging the endocannabinoid signaling system.Journal of neuroscience methods · 2022Review
- Genetically-targeted photorelease of endocannabinoids enables optical control of GPR55 in pancreatic β-cells.Chemical science · 2021Article
- Integrating Endocannabinoid Signalling In Depression.Journal of molecular neuroscience : MN · 2021Review
- Epigenetic Effects of Drugs of Abuse.International journal of environmental research and public health · 2018Review
- Exploiting the Multifaceted Effects of Cannabinoids on Mood to Boost Their Therapeutic Use Against Anxiety and Depression.Frontiers in molecular neuroscience · 2018Article
- Article
- Endocannabinoid modulation of dopamine neurotransmission.Neuropharmacology · 2017Review
- Cannabinoids in Parkinson's Disease.Cannabis and cannabinoid research · 2017Review
- Presynaptic G Protein-Coupled Receptors: Gatekeepers of Addiction?Frontiers in cellular neuroscience · 2016Review
- Ncm, a Photolabile Group for Preparation of Caged Molecules: Synthesis and Biological Application.PloS one · 2016Article
- Cellular Mechanisms of Action of Drug Abuse on Olfactory Neurons.International journal of environmental research and public health · 2015Review
- Endocannabinoids in synaptic plasticity and neuroprotection.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 2015Review
- Diacylglycerol lipase α manipulation reveals developmental roles for intercellular endocannabinoid signaling.Scientific reports · 2013Article
- Endocannabinoid-mediated synaptic plasticity and addiction-related behavior.Neuropharmacology · 2011Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Intercellular signaling dynamics critically influence the functional roles that the signals can play. Small lipids are synthesized and released from neurons, acting as intercellular signals in regulating neurotransmitter release, modulating ion channels on target cells, and modifying synaptic plasticity. The repertoire of biological effects of lipids such as endocannabinoids (eCBs) is rapidly expanding, yet lipid signaling dynamics have not been studied. The eCB system constitutes a powerful tool for bioassaying the dynamics of lipid signaling. The eCBs are synthesized in, and released from, postsynaptic somatodendritic domains that are readily accessible to whole-cell patch electrodes. The dramatic effects of these lipid signals are detected electrophysiologically as CB1-dependent alterations in conventional synaptic transmission, which therefore serve as a sensitive reporter of eCB actions. We used electrophysiological recording, photolytic release of caged glutamate and a newly developed caged AEA (anandamide), together with rapid [Ca2+]i measurements, to investigate the dynamics of retrograde eCB signaling between CA1 pyramidal cells and GABAergic synapses in rat hippocampus in vitro. We show that, at 22 degrees C, eCB synthesis and release must occur within 75-190 ms after the initiating stimulus, almost an order of magnitude faster than previously thought. At 37 degrees C, the time could be < 50 ms. Activation of CB1 and downstream processes constitute a significant fraction of the total delay and are identified as major rate-limiting steps in retrograde signaling. Our findings imply that lipid messenger dynamics are comparable with those of metabotropic neurotransmitters and can modulate neuronal interactions on a similarly fast time scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.