ArticleJournal of cell science2005
Actin and non-muscle myosin II facilitate apical exocytosis of tear proteins in rabbit lacrimal acinar epithelial cells.
Article in Journal of cell science, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 88 citations in OpenAlex.
- Steady-state epithelial apical flatness is characterized by MLCK morphodynamics and asynchronous CaMolecular biology of the cell · 2026Article
- Fusion pore dynamics of large secretory vesicles define a distinct mechanism of exocytosis.The Journal of cell biology · 2023Article
- The miRNA Landscape of Lacrimal Glands in a Murine Model of Autoimmune Dacryoadenitis.Investigative ophthalmology & visual science · 2023Article
- Phenylephrine increases tear cathepsin S secretion in healthy murine lacrimal gland acinar cells through an alternative secretory pathway.Experimental eye research · 2021Article
- Exocytosis by vesicle crumpling maintains apical membrane homeostasis during exocrine secretion.Developmental cell · 2021Article
- Actin and Myosin in Non-Neuronal Exocytosis.Cells · 2020Review
- Cathepsin S activation contributes to elevated CX3CL1 (fractalkine) levels in tears of a Sjögren's syndrome murine model.Scientific reports · 2020Article
- Anaphylactic Degranulation of Mast Cells: Focus on Compound Exocytosis.Journal of immunology research · 2019Review
- The Actomyosin Cytoskeleton Drives Micron-Scale Membrane Remodeling In Vivo Via the Generation of Mechanical Forces to Balance Membrane Tension Gradients.BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- Concerted actions of distinct nonmuscle myosin II isoforms drive intracellular membrane remodeling in live animals.The Journal of cell biology · 2017Article
- Article
- Real-time insights into regulated exocytosis.Journal of cell science · 2017Review
- Labial Salivary Glands in Infants: Histochemical Analysis of Cytoskeletal and Antimicrobial Proteins.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2016Article
- Imbalanced Rab3D versus Rab27 increases cathepsin S secretion from lacrimal acini in a mouse model of Sjögren's Syndrome.American journal of physiology. Cell physiology · 2016Article
- Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.Nature communications · 2015Article
- Imaging membrane remodeling during regulated exocytosis in live mice.Experimental cell research · 2015Review
- Tear-mediated delivery of nanoparticles through transcytosis of the lacrimal gland.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Actin depolymerisation and crosslinking join forces with myosin II to contract actin coats on fused secretory vesicles.Journal of cell science · 2015Article
- A thermo-responsive protein treatment for dry eyes.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Assessing the secretory capacity of pancreatic acinar cells.Journal of visualized experiments : JoVE · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The acinar epithelial cells of the lacrimal gland exocytose the contents of mature secretory vesicles containing tear proteins at their apical membranes in response to secretagogues. Here we use time-lapse confocal fluorescence microscopy and fluorescence recovery after photobleaching to investigate the changes in actin filaments located beneath the apical membrane during exocytosis evoked by the muscarinic agonist, carbachol (100 microM). Time-lapse confocal fluorescence microscopy of apical actin filaments in reconstituted rabbit lacrimal acini transduced with replication-deficient adenovirus containing GFP-actin revealed a relatively quiescent apical actin array in resting acini. Carbachol markedly increased apical actin filament turnover and also promoted transient actin assembly around apparent fusion intermediates. Fluorescence recovery after photobleaching measurements revealed significant (P< or =0.05) increases and decreases, respectively, in mobile fraction (Mf) and turnover times (t1/2) for apical actin filaments in carbachol-stimulated acini relative to untreated acini. The myosin inhibitors, 2,3-butanedione monoxime (BDM, 10 mM, 15 minutes) and ML-7 (40 microM, 15 minutes), significantly decreased carbachol-stimulated secretion of bulk protein and the exogenous secretory vesicle marker, syncollin-GFP; these agents also promoted accumulation of actin-coated structures which were enriched, in transduced acini, in syncollin-GFP, confirming their identity as fusion intermediates. Actin-coated fusion intermediates were sized consistent with incorporation of multiple rather than single secretory vesicles; moreover, BDM and ML-7 caused a shift towards formation of multiple secretory vesicle aggregates while significantly increasing the diameter of actin-coated fusion intermediates. Our findings suggest that the increased turnover of apical actin filaments and the interaction of actin with non-muscle myosin II assembled around aggregates of secretory vesicles facilitate exocytosis in lacrimal acinar epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.