ArticleRespiratory research2005
A confocal microscopic study of solitary pulmonary neuroendocrine cells in human airway epithelium.
Article in Respiratory research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Metastatic neuroendocrine lesions in diffuse idiopathic pulmonary neuroendocrine cell hyperplasia (DIPNECH): a narrative review and case series.Endocrine oncology (Bristol, England) · 2026Review
- Human Pulmonary Neuroendocrine Cells Respond to House Dust Mite Extract With PAR-1 Dependent Release of CGRP.Allergy · 2025Article
- Neuroendocrine cells initiate protective upper airway reflexes.Science (New York, N.Y.) · 2024Article
- Review
- The Dual Role of the Airway Epithelium in Asthma: Active Barrier and Regulator of Inflammation.Cells · 2023Review
- Stem/Progenitor Cells and Related Therapy in Bronchopulmonary Dysplasia.International journal of molecular sciences · 2023Review
- Article
- Neuro-Immune Regulation in Inflammation and Airway Remodeling of Allergic Asthma.Frontiers in immunology · 2022Review
- Calcitonin-secreting neuroendocrine neoplasms of the lung: a systematic review and narrative synthesis.Endocrine connections · 2021Article
- Airway basal stem cells generate distinct subpopulations of PNECs.Cell reports · 2021Article
- Stem cells and lung regeneration.American journal of physiology. Cell physiology · 2020Review
- Cell-specific expression of lung disease risk-related genes in the human small airway epithelium.Respiratory research · 2020Article
- Efficient Generation and Transcriptomic Profiling of Human iPSC-Derived Pulmonary Neuroendocrine Cells.iScience · 2020Article
- Neuropeptides in asthma, chronic obstructive pulmonary disease and cystic fibrosis.Respiratory research · 2018Review
- Neuropeptides SP and CGRP Diminish theMediators of inflammation · 2018Article
- Co-expression of Achaete-Scute Homologue-1 and Calcitonin Gene-Related Peptide during NNK-Induced Pulmonary Neuroendocrine Hyperplasia and Carcinogenesis in Hamsters.Journal of Cancer · 2016Article
- Morphologic Characterization of Nerves in Whole-Mount Airway Biopsies.American journal of respiratory and critical care medicine · 2015Article
- Chemosensory functions for pulmonary neuroendocrine cells.American journal of respiratory cell and molecular biology · 2014Article
- Detection and organ-specific ablation of neuroendocrine cells by synaptophysin locus-based BAC cassette in transgenic mice.PloS one · 2013Article
- Unmyelinated nerve fibers in the human dental pulp express markers for myelinated fibers and show sodium channel accumulations.BMC neuroscience · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPulmonary neuroendocrine cells (PNEC) are specialized epithelial cells that are thought to play important roles in lung development and airway function. PNEC occur either singly or in clusters called neuroepithelial bodies. Our aim was to characterize the three dimensional morphology of PNEC, their distribution, and their relationship to the epithelial nerves in whole mounts of adult human bronchi using confocal microscopy.
methodsBronchi were resected from non-diseased portions of a lobe of human lung obtained from 8 thoracotomy patients (Table 1) undergoing surgery for the removal of lung tumors. Whole mounts were stained with antibodies to reveal all nerves (PGP 9.5), sensory nerves (calcitonin gene related peptide, CGRP), and PNEC (PGP 9.5, CGRP and gastrin releasing peptide, GRP). The analysis and rendition of the resulting three-dimensional data sets, including side-projections, was performed using NIH-Image software. Images were colorized and super-imposed using Adobe Photoshop.
resultsPNEC were abundant but not homogenously distributed within the epithelium, with densities ranging from 65/mm2 to denser patches of 250/mm2, depending on the individual wholemount. Rotation of 3-D images revealed a complex morphology; flask-like with the cell body near the basement membrane and a thick stem extending to the lumen. Long processes issued laterally from its base, some lumenal and others with feet-like processes. Calcitonin gene-related peptide (CGRP) was present in about 20% of PNEC, mainly in the processes. CGRP-positive nerves were sparse, with some associated with the apical part of the PNEC.
conclusionOur 3D-data demonstrates that PNEC are numerous and exhibit a heterogeneous peptide content suggesting an active and diverse PNEC population.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.