ArticleHistochemistry and cell biology2006
Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine.
Article in Histochemistry and cell biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 184 papers.
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Who cites it
184 citing papers in PubMed, 379 citations in OpenAlex.
- Cyclo-C stabilizes PEX13 to inhibit porcine epidemic diarrhea virus replication by blocking pexophagy-mediated disruption of antiviral innate immunity.Journal of virology · 2026Article
- Cell Culture Adaptation of Porcine Group A Rotavirus: Advances and Challenges for Vaccine Development.Viruses · 2026Review
- The Pro-Inflammatory Action of Different Pathogen-Associated Molecular Patterns in Porcine Hepatic and Intestinal Cell Cultures.Animals : an open access journal from MDPI · 2026Article
- Culture Optimization of the IPEC-J2 Piglet Jejunum Cell Line for Applications in Infant Nutrition Research.Molecular nutrition & food research · 2026Article
- Investigating alterations associated with heat stress and the recovery of the intestinal barrier using IPEC-J2 as an intestinal epithelial porcine cell model.Scientific reports · 2026Article
- Protective Effects of Humic Acid on Intestinal Barrier Dysfunction and Inflammatory Activation in Canine Cell-Based Models.Animals : an open access journal from MDPI · 2026Article
- Porcine epidemic diarrhea virus induces Vero cell mitophagy by regulating ROS-mediated PINK1/Parkin signal pathway.Frontiers in veterinary science · 2026Article
- Article
- IPEC-J2 as a cellular model for studying intestinal mucus.Scientific reports · 2025Article
- Relevance of porcine intestinal organoids as a surrogate for animal experimentation: application to the investigation of host-virus interactions during porcine coronavirus infection.Veterinary research · 2025Article
- Integrated multi-omics reveals the Bacillus amyloliquefaciens BA40 against Clostridium perfringens infection in weaned piglets.Journal of advanced research · 2025Article
- Butyrate Derivatives Exhibited Anti-Inflammatory Effects and Enhanced Intestinal Barrier Integrity in Porcine Cell Culture Models.Animals : an open access journal from MDPI · 2025Article
- Single-cell transcriptomics predict novel potential regulators of acute epithelial restitution in the ischemia-injured intestine.American journal of physiology. Gastrointestinal and liver physiology · 2025Article
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- The Efficacy of a Ferric Sillen Core-Linked Polymer in Suppressing the Pathogenicity ofAnimals : an open access journal from MDPI · 2024Article
- A NovelAntibiotics (Basel, Switzerland) · 2024Article
- In Vitro Evaluation of Antipseudomonal Activity and Safety Profile of Peptidomimetic Furin Inhibitors.Biomedicines · 2024Article
- A Quantity-Dependent Nonlinear Model of Sodium Cromoglycate Suppression on Beta-Conglycinin Transport.International journal of molecular sciences · 2024Article
- Impact of Nutritional Strategies to Prevent Post-Weaning Diarrhoea on Performance, Behaviour, and Microbiota in Piglets from Organic Farming.Animals : an open access journal from MDPI · 2024Article
- Effects of Transportation on Blood Indices, Oxidative Stress, Rumen Fermentation Parameters and Rumen Microbiota in Goats.Animals : an open access journal from MDPI · 2024Article
124 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro studies on the pathogenesis in swine have been hampered by the lack of relevant porcine cell lines. Since many bacterial infections are swine-specific, studies on pathogenic mechanisms require appropriate cell lines of porcine origin. We have characterized the permanent porcine intestinal epithelial cell line, IPEC-J2, using a variety of methods in order to assess the usefulness of this cell line as an in vitro infection model. Electron microscopic analyses and histochemical staining revealed the cells to be enterocyte-like with microvilli, tight junctions and glycocalyx-bound mucin. The functional integrity of monolayers was determined by transepithelial electrical resistance (TEER) measurements. Both commensal bacteria and important bacterial pathogens were chosen for study based on their principally different infection mechanisms: obligate extracellular Escherichia coli, facultative intracellular Salmonella and obligate intracellular Chlamydia. We determined the colonization and proliferation of the bacteria on and within the host cells and monitored the host cell response. We verified the expression of mRNAs encoding the cytokines IL-1alpha, -6, -7, -8, -18, TNF-alpha and GM-CSF, but not TGF-beta or MCP-1. IL-8 protein expression was enhanced by Salmonella invasion. We conclude that the IPEC-J2 cell line provides a relevant in vitro model system for porcine intestinal pathogen-host cell interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.