ArticleEukaryotic cell2005
The Candida albicans vacuole is required for differentiation and efficient macrophage killing.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 62 citations in OpenAlex.
- The Vacuolar Protein 8 (Vac8) Homolog inJournal of fungi (Basel, Switzerland) · 2025Article
- Vacuolar Proteases ofJournal of fungi (Basel, Switzerland) · 2025Article
- The vacuolar fusion regulated by HOPS complex promotes hyphal initiation and penetration in Candida albicans.Nature communications · 2024Article
- Understanding the molecular mechanisms of human diseases: the benefits of fission yeasts.Microbial cell (Graz, Austria) · 2024Article
- The mRNA stability factor Khd4 defines a specific mRNA regulon for membrane trafficking in the pathogenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- A C. albicans TRAPP Complex-Associated Gene Contributes to Cell Wall Integrity, Hyphal and Biofilm Formation, and Tissue Invasion.Microbiology spectrum · 2023Article
- Characterization of Aspartic Proteases fromJournal of fungi (Basel, Switzerland) · 2023Article
- 2,4-Diacetylphloroglucinol ModulatesJournal of fungi (Basel, Switzerland) · 2022Article
- Magnesium impairs Candida albicans immune evasion by reduced hyphal damage, enhanced β-glucan exposure and altered vacuole homeostasis.PloS one · 2022Article
- Functional connections between cell cycle and proteostasis in the regulation of Candida albicans morphogenesis.Cell reports · 2021Review
- The Importance of Vacuolar Ion Homeostasis and Trafficking in Hyphal Development and Virulence inFrontiers in microbiology · 2021Review
- Immunopathology of Recurrent Vulvovaginal Infections: New Aspects and Research Directions.Frontiers in immunology · 2019Review
- pH-Dependant Antifungal Activity of Valproic Acid against the Human Fungal PathogenFrontiers in microbiology · 2017Article
- Identification of small molecules that disrupt vacuolar function in the pathogen Candida albicans.PloS one · 2017Article
- ERG2 and ERG24 Are Required for Normal Vacuolar Physiology as Well as Candida albicans Pathogenicity in a Murine Model of Disseminated but Not Vaginal Candidiasis.Eukaryotic cell · 2015Article
- The Candida albicans Exocyst Subunit Sec6 Contributes to Cell Wall Integrity and Is a Determinant of Hyphal Branching.Eukaryotic cell · 2015Article
- Trafficking through the late endosome significantly impacts Candida albicans tolerance of the azole antifungals.Antimicrobial agents and chemotherapy · 2015Article
- Intracellular aspartic proteinase Apr1p of Candida albicans is required for morphological transition under nitrogen-limited conditions but not for macrophage killing.Folia microbiologica · 2014Article
- The contribution of Candida albicans vacuolar ATPase subunit V₁B, encoded by VMA2, to stress response, autophagy, and virulence is independent of environmental pH.Eukaryotic cell · 2014Article
- Candida albicans VPS4 contributes differentially to epithelial and mucosal pathogenesis.Virulence · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Yeast-hypha differentiation is believed to be necessary for the normal progression of Candida albicans infections. The emergence and extension of a germ tube from a parental yeast cell are accompanied by dynamic changes in vacuole size and morphology. Although vacuolar function is required during this process, it is unclear if it is vacuolar expansion or some other vacuolar function that is important. We previously described a C. albicans vps11Delta mutant which lacked a recognizable vacuole compartment and with defects in multiple vacuolar functions. These include sensitivities to stress, reduced proteolytic activities, and severe defects in filamentation. Herein we utilize a partially functional VPS11 allele (vps11hr) to help define which vacuolar functions are required for differentiation and which influence interaction with macrophages. Mutant strains harboring this allele are not osmotically or temperature sensitive and have normal levels of secreted aspartyl protease and carboxypeptidase Y activity but have a fragmented vacuole morphology. Moreover, this mutant is defective in filamentation, suggesting that the major role the vacuole plays in yeast-hypha differentiation may relate directly to its morphology. The results of this study support the hypothesis that vacuole expansion is required during germ tube emergence. Both vps11 mutants were severely attenuated in their ability to kill a macrophage cell line. The viability of the vps11delta mutant was significantly reduced during macrophage interaction compared to that in the control strains, while the vps11hr mutant was unaffected. This implies some vacuolar functions are required for Candida survival within the macrophage, while additional vacuolar functions are required to inflict injury on the macrophage.
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