ArticleThe Plant cell2005
Ectopic DICER-LIKE1 expression in P1/HC-Pro Arabidopsis rescues phenotypic anomalies but not defects in microRNA and silencing pathways.
Article in The Plant cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 74 citations in OpenAlex.
- HC-Pro inhibits HEN1 methyltransferase activity, leading to autophagic degradation of AGO1.Nature communications · 2025Article
- RNA Interference: Promising Approach to Combat Plant Viruses.International journal of molecular sciences · 2022Review
- Small RNAs Participate in Plant-Virus Interaction and Their Application in Plant Viral Defense.International journal of molecular sciences · 2022Review
- DEAD-box helicases modulate dicing body formation inScience advances · 2021Article
- Association between flower stalk elongation, an Arabidopsis developmental trait, and the subcellular location and movement dynamics of the nonstructural protein P3 of Turnip mosaic virus.Molecular plant pathology · 2020Article
- EngineeringPlant direct · 2020Article
- Characterization of Maize miRNAs in Response to Synergistic Infection of Maize Chlorotic Mottle Virus and Sugarcane Mosaic Virus.International journal of molecular sciences · 2019Article
- Review
- RNA decay is an antiviral defense in plants that is counteracted by viral RNA silencing suppressors.PLoS pathogens · 2018Article
- The HCPro from the Potyviridae family: an enviable multitasking Helper Component that every virus would like to have.Molecular plant pathology · 2018Review
- Expression analysis of argonaute, Dicer-like, and RNA-dependent RNA polymerase genes in cucumber (Cucumis sativus L.) in response to abiotic stress.Journal of genetics · 2017Article
- Developmental Defects Mediated by the P1/HC-Pro Potyviral Silencing Suppressor Are Not Due to Misregulation of AUXIN RESPONSE FACTOR 8.Plant physiology · 2016Article
- Gene Perturbation Atlas (GPA): a single-gene perturbation repository for characterizing functional mechanisms of coding and non-coding genes.Scientific reports · 2015Article
- Plum pox virus and sharka: a model potyvirus and a major disease.Molecular plant pathology · 2014Review
- Unrelated viral suppressors of RNA silencing mediate the control of ARGONAUTE1 level.Molecular plant pathology · 2013Article
- The helicase and RNaseIIIa domains of Arabidopsis Dicer-Like1 modulate catalytic parameters during microRNA biogenesis.Plant physiology · 2012Article
- Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.PLoS pathogens · 2011Article
- HC-Pro silencing suppressor significantly alters the gene expression profile in tobacco leaves and flowers.BMC plant biology · 2011Article
- Optimal viral strategies for bypassing RNA silencing.Journal of the Royal Society, Interface · 2011Article
- Multiple functions of Rice dwarf phytoreovirus Pns10 in suppressing systemic RNA silencing.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the viral silencing suppressor P1/HC-Pro in plants causes severe developmental anomalies accompanied by defects in both short interfering RNA (siRNA) and microRNA (miRNA) pathways. P1/HC-Pro transgenic lines fail to accumulate the siRNAs that mediate RNA silencing and are impaired in both miRNA processing and function, accumulating abnormally high levels of miRNA/miRNA* processing intermediates as well as miRNA target messages. Both miRNA and RNA silencing pathways require participation of DICER-LIKE (DCL) ribonuclease III-like enzymes. Here, we investigate the effects of overexpressing DCL1, one of four Dicers in Arabidopsis thaliana, on P1/HC-Pro-induced defects in development and small RNA metabolism. Expression of a DCL1 cDNA transgene (35S:DCL1) produced a mild gain-of-function phenotype and largely rescued dcl1 mutant phenotypes. The 35S:DCL1 plants were competent for virus-induced RNA silencing but were impaired in transgene-induced RNA silencing and in the accumulation of some miRNAs. Ectopic DCL1 largely alleviated developmental anomalies in P1/HC-Pro plants but did not correct the P1/HC-Pro-associated defects in small RNA pathways. The ability of P1/HC-Pro plants to suppress RNA silencing and the levels of miRNAs, miRNA*s, and miRNA target messages in these plants were essentially unaffected by ectopic DCL1. These data suggest that P1/HC-Pro defects in development do not result from general impairments in small RNA pathways and raise the possibility that DCL1 participates in processes in addition to miRNA biogenesis.
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