ArticleBiophysical journal2006
Lipid asymmetry in DLPC/DSPC-supported lipid bilayers: a combined AFM and fluorescence microscopy study.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Engineered Hybrid Vesicles and Cellular Internalization in Mammary Cancer Cells.Pharmaceutics · 2024Article
- Domain Localization by Graphene Oxide in Supported Lipid Bilayers.International journal of molecular sciences · 2023Article
- Single-Molecule Trapping and Measurement in a Nanostructured Lipid Bilayer System.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Psychotropic drugs interaction with the lipid nanoparticle of COVID-19 mRNA therapeutics.Frontiers in pharmacology · 2022Article
- Influenza A virus hemagglutinin prevents extensive membrane damage upon dehydration.BBA advances · 2022Article
- Hydration Layer of Only a Few Molecules Controls Lipid Mobility in Biomimetic Membranes.Journal of the American Chemical Society · 2021Article
- Fabrication and electromechanical characterization of freestanding asymmetric membranes.Biophysical journal · 2021Article
- Polar Lipid Fraction E fromInternational journal of molecular sciences · 2020Article
- Flowering Poration-A Synergistic Multi-Mode Antibacterial Mechanism by a Bacteriocin Fold.iScience · 2020Article
- To Hop or not to Hop: Exceptions in the FCS Diffusion Law.Biophysical journal · 2020Article
- Recent Applications of Advanced Atomic Force Microscopy in Polymer Science: A Review.Polymers · 2020Review
- Plasma membrane asymmetry of lipid organization: fluorescence lifetime microscopy and correlation spectroscopy analysis.Journal of lipid research · 2020Article
- Interleaflet Coupling of Lipid Nanodomains - Insights FromFrontiers in cell and developmental biology · 2020Review
- Do ABC transporters regulate plasma membrane organization?Cellular & molecular biology letters · 2020Review
- Atomic force microscopy of phase separation on ruptured, giant unilamellar vesicles, and a mechanical pathway for the co-existence of lipid gel phases.Journal of biomechanical engineering · 2019Article
- Effects of Passive Phospholipid Flip-Flop and Asymmetric External Fields on Bilayer Phase Equilibria.Biophysical journal · 2018Article
- Asymmetric Hybrid Polymer-Lipid Giant Vesicles as Cell Membrane Mimics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Antimicrobial peptide capsids of de novo design.Nature communications · 2017Article
- Engineering monolayer poration for rapid exfoliation of microbial membranes.Chemical science · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A fundamental attribute of cell membranes is transmembrane asymmetry, specifically the formation of ordered phase domains in one leaflet that are compositionally different from the opposing leaflet of the bilayer. Using model membrane systems, many previous studies have demonstrated the formation of ordered phase domains that display complete transmembrane symmetry; but there have been few reports on the more biologically relevant asymmetric membrane structures. Here we report on a combined atomic force microscopy and fluorescence microscopy study whereby we observe three different states of transmembrane symmetry in phase-separated supported lipid bilayers formed by vesicle fusion. We find that if the leaflets differ in gel-phase area fraction, then the smaller domains in one leaflet are in registry with the larger domains in the other leaflet and the system is dynamic. In a presumed lipid flip-flop process similar to Ostwald ripening, the smaller domains in one leaflet erode away whereas the large domains in the other leaflet grow until complete compositional asymmetry is reached and remains stable. We have quantified this evolution and determined that the lipid flip-flop event happens most frequently at the interface between symmetric and asymmetric DSPC domains. If both leaflets have identical area fraction of gel-phase, gel-phase domains are in registry and are static in comparison to the first state. The stability of these three DSPC domain distributions, the degree of registry observed, and the domain immobility have biological significance with regards to maintenance of lipid asymmetry in living cell membranes, communication between inner leaflet and outer leaflet, membrane adhesion, and raft mobility.
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