ArticleNucleic acids research2005
Dominant-negative mutant phenotypes and the regulation of translation elongation factor 2 levels in yeast.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Emw1/TTC27 is a chaperone required for folding of the eukaryotic elongation factor 2.Cellular and molecular life sciences : CMLS · 2026Article
- Review
- Hsp90 and cochaperones have two genetically distinct roles in regulating eEF2 function.PLoS genetics · 2024Article
- eEF2 diphthamide modification restrains spurious frameshifting to maintain translational fidelity.Nucleic acids research · 2023Article
- Spinocerebellar ataxias (SCAs) caused by common mutations.Neurogenetics · 2021Review
- Reciprocal regulation of hnRNP C and CELF2 through translation and transcription tunes splicing activity in T cells.Nucleic acids research · 2020Article
- Importance of diphthamide modified EF2 for translational accuracy and competitive cell growth in yeast.PloS one · 2018Article
- In Vivo Translatome Profiling in Spinal Muscular Atrophy Reveals a Role for SMN Protein in Ribosome Biology.Cell reports · 2017Article
- Mutations in the G-domain of Ski7 cause specific dysfunction in non-stop decay.Scientific reports · 2016Article
- Review
- Pseudomonas Exotoxin A: optimized by evolution for effective killing.Frontiers in microbiology · 2015Review
- The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network.PLoS genetics · 2013Article
- A conserved eEF2 coding variant in SCA26 leads to loss of translational fidelity and increased susceptibility to proteostatic insult.Human molecular genetics · 2012Article
- eIF5A interacts functionally with eEF2.Amino acids · 2012Article
- A Toxoplasma gondii mutant highlights the importance of translational regulation in the apicoplast during animal infection.Molecular microbiology · 2011Article
- Yeast 18 S rRNA is directly involved in the ribosomal response to stringent AUG selection during translation initiation.The Journal of biological chemistry · 2010Article
- Selection systems based on dominant-negative transcription factors for precise genetic engineering.Nucleic acids research · 2010Article
- The role of protein synthesis in cell cycling and cancer.Molecular oncology · 2009Review
- Article
- Functional characterization of ribosomal protein L15 from Saccharomyces cerevisiae.Current genetics · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The eukaryotic translation elongation factor 2 (eEF2), a member of the G-protein superfamily, catalyzes the post-peptidyl transferase translocation of deacylated tRNA and peptidyl tRNA to the ribosomal E- and P-sites. eEF2 is modified by a unique post-translational modification: the conversion of His699 to diphthamide at the tip of domain IV, the region proposed to mimic the anticodon of tRNA. Structural models indicate a hinge is important for conformational changes in eEF2. Mutations of V488 in the hinge region and H699 in the tip of domain IV produce non-functional mutants that when co-expressed with the wild-type eEF2 result in a dominant-negative growth phenotype in the yeast Saccharomyces cerevisiae. This phenotype is linked to reduced levels of the wild-type protein, as total eEF2 levels are unchanged. Changes in the promoter, 5'-untranslated region (5'-UTR) or 3'-UTR of the EFT2 gene encoding eEF2 do not allow overexpression of the protein, showing that eEF2 levels are tightly regulated. The H699K mutant, however, also alters translation phenotypes. The observed regulation suggests that the cell needs an optimum amount of active eEF2 to grow properly. This provides information about a new mechanism by which translation is efficiently maintained.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.