ArticleFEMS microbiology letters2005
Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae.
Article in FEMS microbiology letters, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- A genetic manipulation tool based on the GP35 recombinase for targeted gene editing in mycoplasmas of ruminants.The Journal of biological chemistry · 2026Article
- Mycoplasma bovis 5'-nucleotidase is a virulence factor conferring mammary fitness in bovine mastitis.PLoS pathogens · 2024Article
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- Lipoproteins Are Potent Activators of Nuclear Factor Kappa B in Mammary Epithelial Cells and Virulence Factors inMicroorganisms · 2022Article
- Development of oriC-plasmids for use in Mycoplasma hyorhinis.Scientific reports · 2017Article
- Vpma phase variation is important for survival and persistence of Mycoplasma agalactiae in the immunocompetent host.PLoS pathogens · 2017Article
- Development ofApplied and environmental microbiology · 2017Article
- Simultaneous Identification of Potential Pathogenicity Factors of Mycoplasma agalactiae in the Natural Ovine Host by Negative Selection.Infection and immunity · 2015Article
- Article
- Development and host compatibility of plasmids for two important ruminant pathogens, Mycoplasma bovis and Mycoplasma agalactiae.PloS one · 2015Article
- In vitro and in vivo cell invasion and systemic spreading of Mycoplasma agalactiae in the sheep infection model.International journal of medical microbiology : IJMM · 2014Article
- Development of a self-replicating plasmid system for Mycoplasma hyopneumoniae.Veterinary research · 2013Article
- Development of a site-directed integration plasmid for heterologous gene expression in Mycoplasma gallisepticum.PloS one · 2013Article
- Complete genome sequence of Mycoplasma suis and insights into its biology and adaption to an erythrocyte niche.PloS one · 2011Article
- Ureaplasma antigenic variation beyond MBA phase variation: DNA inversions generating chimeric structures and switching in expression of the MBA N-terminal paralogue UU172.Molecular microbiology · 2011Article
- Genome-scale analysis of Mycoplasma agalactiae loci involved in interaction with host cells.PloS one · 2011Article
- Enhancement of targeted homologous recombination in Mycoplasma mycoides subsp. capri by inclusion of heterologous recA.Applied and environmental microbiology · 2010Article
- Targeted chromosomal knockouts in Mycoplasma pneumoniae.Applied and environmental microbiology · 2010Article
- Critical role of dispensable genes in Mycoplasma agalactiae interaction with mammalian cells.Infection and immunity · 2010Article
- Phase-locked mutants of Mycoplasma agalactiae: defining the molecular switch of high-frequency Vpma antigenic variation.Molecular microbiology · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic tools has been a major impediment in studying the pathogenic mechanisms of M. agalactiae. This study describes the identification and cloning of the M. agalactiae origin of replication (oriC) in order to construct the first shuttle vectors for targeted gene disruption, gene complementation and expression studies. Additionally, this report provides the first evidence of the occurrence of homologous recombination and the functionality of heterologous tetM determinant in this pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.