ArticleJournal of structural and functional genomics2005
Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production.
Article in Journal of structural and functional genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Comparison of different transient gene expression systems for the production of a new humanized anti-HER2 monoclonal antibody (Hersintuzumab).Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2023Article
- Article
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- Article
- Augmenting recombinant antibody production in HEK293E cells: optimizing transfection and culture parameters.Antibody therapeutics · 2022Article
- New-Generation Benzimidazole-Based Plasmid Delivery Reagents with High Transfection Efficiencies on the Mammalian Cells.In vitro cellular & developmental biology. Animal · 2020Article
- Functional Analysis of the 3' Untranslated Region of the HumanNeuropsychiatric disease and treatment · 2020Article
- Design and structural characterisation of olfactomedin-1 variants as tools for functional studies.BMC molecular and cell biology · 2019Article
- Article
- Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering.Journal of biological engineering · 2015Article
- Optimizing the transient transfection process of HEK-293 suspension cells for protein production by nucleotide ratio monitoring.Cytotechnology · 2014Article
- Recent advances in mammalian protein production.FEBS letters · 2014Review
- High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells.BMC biotechnology · 2013Article
- Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.Current opinion in structural biology · 2013Review
- Ebola virus VP35 induces high-level production of recombinant TPL-2-ABIN-2-NF-κB1 p105 complex in co-transfected HEK-293 cells.The Biochemical journal · 2013Article
- Methadone but not morphine inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents.Cell biochemistry and biophysics · 2013Article
- Expression of recombinant antibodies.Frontiers in immunology · 2013Article
- Expression of SARS-coronavirus spike glycoprotein in Pichia pastoris.Virus genes · 2009Article
- Transient gene delivery for functional enrichment of differentiating embryonic stem cells.Biotechnology and bioengineering · 2008Article
- Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian expression systems have an undisputed long-standing and very successful history for the generation of recombinant proteins, mainly as biopharmaceuticals. However, for use as 'tool proteins' in, e.g. assay development and screening, for structure elucidation and as antigens these expression systems were generally regarded as being cumbersome, tedious and expensive. This bias has largely been overcome with the very recent development of large-scale transient transfection (LST) approaches. Especially the HEK.EBNA expression system described here has contributed significantly to this success. The simplicity and speed of this approach compares well with expression trials using the widely applied Baculovirus/insect cell system. In addition, proteins generated in mammalian cells are usually correctly folded, fully processed and functionally active.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.