ArticleJournal of structural and functional genomics2005
Large-scale expression and purification of a G-protein-coupled receptor for structure determination -- an overview.
Article in Journal of structural and functional genomics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed.
- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Efficient production of a functional G protein-coupled receptor in E. coli for structural studies.Journal of biomolecular NMR · 2021Article
- Production of G protein-coupled receptors in an insect-based cell-free system.Biotechnology and bioengineering · 2017Article
- Kinetics of the early events of GPCR signalling.FEBS letters · 2014Article
- Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Tolerance to changes in membrane lipid composition as a selected trait of membrane proteins.Biochemistry · 2011Review
- Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents.Protein science : a publication of the Protein Society · 2011Article
- Lipid and peptide dynamics in membranes upon insertion of n-alkyl-beta-D-glucopyranosides.Biophysical journal · 2010Article
- Surface plasmon resonance for high-throughput ligand screening of membrane-bound proteins.Biotechnology journal · 2009Review
- Structure of a double transmembrane fragment of a G-protein-coupled receptor in micelles.Biophysical journal · 2009Article
- An overview on GPCRs and drug discovery: structure-based drug design and structural biology on GPCRs.Methods in molecular biology (Clifton, N.J.) · 2009Review
- High-throughput T7 LIC vector for introducing C-terminal poly-histidine tags with variable lengths without extra sequences.Protein expression and purification · 2009Article
- High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1.PloS one · 2009Article
- Biosynthesis and NMR-studies of a double transmembrane domain from the Y4 receptor, a human GPCR.Journal of biomolecular NMR · 2008Article
- Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Analysis of a G protein-coupled receptor for neurotensin by liquid chromatography-electrospray ionization-mass spectrometry.Analytical biochemistry · 2008Article
- Mapping the membrane-aqueous border for the voltage-sensing domain of a potassium channel.The Journal of biological chemistry · 2007Article
- NMR studies in dodecylphosphocholine of a fragment containing the seventh transmembrane helix of a G-protein-coupled receptor from Saccharomyces cerevisiae.Biophysical journal · 2007Article
- Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein.Protein expression and purification · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance studies, require milligram quantities of purified, functional receptor protein on a regular basis. We present an overview on expression and purification studies with a receptor for neurotensin. Functional expression in Escherichia coli and an automated two-column purification routine allow ongoing crystallization experiments and studies on receptor-bound ligands.
Indexed as
Identifiers
16211513What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.