ArticleJournal of clinical microbiology2005
Development of PCR assays targeting the genes involved in synthesis and assembly of the new Escherichia coli O 174 and O 177 O antigens.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Potential Zoonotic Pathovars of DiarrheagenicMicroorganisms · 2021Article
- The First Isolation and Molecular Characterization of Shiga Toxin-Producing Virulent Multi-Drug Resistant Atypical EnteropathogenicFrontiers in cellular and infection microbiology · 2019Article
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- oqxAB encoding a multidrug efflux pump in human clinical isolates of Enterobacteriaceae.Antimicrobial agents and chemotherapy · 2009Article
- A new O-antigen gene cluster has a key role in the virulence of the Escherichia coli meningitis clone O45:K1:H7.Journal of bacteriology · 2007Article
- Tracing shigatoxigenic Escherichia coli O103, O145, and O174 infections from farm residents to cattle.Journal of clinical microbiology · 2007Article
- Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli.Journal of bacteriology · 2007Article
- Identification of sequence diversity in the Escherichia coli fliC genes encoding flagellar types H8 and H40 and its use in typing of Shiga toxin-producing E. coli O8, O22, O111, O174, and O179 strains.Journal of clinical microbiology · 2007Article
- Development of a serotype-specific DNA microarray for identification of some Shigella and pathogenic Escherichia coli strains.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli O 174 and O 177 are newly described O serogroups which were reported as human pathogens. Identification of these strains by serotyping has been restricted, as the required sera are not commercially available. In this study, a collection of 13 E. coli O 174 strains and 12 E. coli O 177 strains was studied on the O:H serotypes and virulence markers. The O-antigen gene clusters of E. coli O 174 and O 177 were sequenced, and associated genes were assigned functions on the basis of homology. Two genes, each specific for E. coli O 174 and O 177, were identified. PCR assays based on the O-antigen-specific genes were developed and tested on 25 clinical and environmental isolates of those two serogroups as well as 26 isolates of other O serogroups. As little as 1 pg per mul of chromosomal DNA and as few as 0.1 CFU per g of pork and water samples were detected for either strain. The PCR assays established in this study were shown to be highly sensitive and reliable and could be the method of choice for detection of these two human pathogens from clinical, food, and other environmental samples.
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