ArticleJournal of clinical microbiology2005
Development of multiplex PCRs for detection of common viral pathogens and agents of congenital infections.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Antenatal interventions for preventing the transmission of cytomegalovirus (CMV) from the mother to fetus during pregnancy and adverse outcomes in the congenitally infected infant.The Cochrane database of systematic reviews · 2011Pooled it
- DNA Polymerases for Whole Genome Amplification: Considerations and Future Directions.International journal of molecular sciences · 2023Review
- Molecular Mechanisms behind Persistent Presence of Parvovirus B19 in Human Dilated Myocardium.Advances in experimental medicine and biology · 2022Article
- Prevalence and prognostic relevance of myocardial inflammation and cardiotropic viruses in non-ischemic dilated cardiomyopathy.Cardiology journal · 2022Article
- Next generation sequencing of human enterovirus strains from an outbreak of enterovirus A71 shows applicability to outbreak investigations.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- The Association Between Documentation of Koplik Spots and Laboratory Diagnosis of Measles and Other Rash Diseases in a National Measles Surveillance Program in Japan.Frontiers in microbiology · 2019Article
- Article
- Pathogen profiles and molecular epidemiology of respiratory viruses in Japanese inpatients with community-acquired pneumonia.Respiratory investigation · 2016Observational
- The immunological underpinnings of vaccinations to prevent cytomegalovirus disease.Cellular & molecular immunology · 2015Review
- Congenital cytomegalovirus infection: Clinical presentation, epidemiology, diagnosis and prevention.Obstetric medicine · 2014Review
- Development and padronization of three multiplex PCRs for the diagnosis of Chlamydia trachomatis, Toxoplasma gondii, herpes simplex viruses 1 and 2, and Cytomegalovirus.Molecular biotechnology · 2013Article
- Detection of human herpesvirus 8 by quantitative polymerase chain reaction: development and standardisation of methods.BMC infectious diseases · 2012Article
- Children with islet autoimmunity and enterovirus infection demonstrate a distinct cytokine profile.Diabetes · 2012Article
- Immunology in the clinic review series; focus on type 1 diabetes and viruses: the enterovirus link to type 1 diabetes: critical review of human studies.Clinical and experimental immunology · 2012Review
- Comparison of DNA extraction methods from small samples of newborn screening cards suitable for retrospective perinatal viral research.Journal of biomolecular techniques : JBT · 2011Article
- Multiplex PCR testing detection of higher-than-expected rates of cervical mycoplasma, ureaplasma, and trichomonas and viral agent infections in sexually active australian women.Journal of clinical microbiology · 2009Article
- Large scale multiplex PCR improves pathogen detection by DNA microarrays.BMC microbiology · 2009Article
- Etiological Profile and Clinico Epidemiological Patterns of Acute Encephalitis Syndrome in Tamil Nadu, India.Journal of global infectious diseasesArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus. Testing for each of these agents using nucleic acid tests is time consuming and the availability of clinical samples such as amniotic fluid or neonatal blood is often limited. The aim of this study was to develop multiplex PCRs (mPCRs) for detection of DNA and RNA agents in the investigation of congenital infection and an mPCR for the viruses most commonly requested in a diagnostic virology laboratory (CMV, Epstein-Barr virus, enterovirus, HSV-1, HSV-2, and varicella-zoster virus). The assays were assessed using known pathogen-positive tissues (cultures, placentae, plasma, and amniotic fluid) and limits of detection were determined for all the agents studied using serial dilutions of plasmid targets. Nested PCR was performed as the most sensitive assay currently available, and detection of the amplicons using hybridization to labeled probes and enzyme-linked immunosorbent assay detection was incorporated into three of the four assays. This allowed detection of 10 to 10(2) copies of each agent in the samples processed. In several patients, an unexpected infection was diagnosed, including a case of encephalitis where HSV was the initial clinical suspicion but CMV was detected. In the majority of these cases the alternative agent could be confirmed using reference culture, serology, or fluorescence methods and was of relevance to clinical care of the patient. The methods described here provide useful techniques for diagnosing congenital infections and a paradigm for assessment of new multiplex PCRs for use in the diagnostic laboratory.
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