ArticleJournal of clinical microbiology2005
Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- PCR in diagnosis of invasive aspergillosis: a meta-analysis of diagnostic performance.Journal of clinical microbiology · 2014Pooled it
- Local retrospective analysis of galactomannan cut-off values in bronchoalveolar lavage fluids for diagnosis of invasive aspergillosis.Folia microbiologica · 2018Article
- CirculatingFrontiers in microbiology · 2017Article
- Comparison of Nonculture Blood-Based Tests for Diagnosing Invasive Aspergillosis in an Animal Model.Journal of clinical microbiology · 2016Article
- Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative.Journal of clinical microbiology · 2015Article
- Combining standard clinical methods with PCR showed improved diagnosis of invasive pulmonary aspergillosis in patients with hematological malignancies and prolonged neutropenia.BMC infectious diseases · 2015Observational
- Detection of multiple fungal species in blood samples by real-time PCR: an interpretative challenge.Journal of clinical microbiology · 2014Article
- Molecular and nonmolecular diagnostic methods for invasive fungal infections.Clinical microbiology reviews · 2014Review
- Multicenter comparison of serum and whole-blood specimens for detection of Aspergillus DNA in high-risk hematological patients.Journal of clinical microbiology · 2013Article
- Laboratory diagnosis of invasive aspergillosis: from diagnosis to prediction of outcome.Scientifica · 2013Review
- A novel extraction method combining plasma with a whole-blood fraction shows excellent sensitivity and reproducibility for patients at high risk for invasive aspergillosis.Journal of clinical microbiology · 2012Article
- Prospective evaluation of clinical and biological markers to predict the outcome of invasive pulmonary aspergillosis in hematological patients.Journal of clinical microbiology · 2012Article
- Evaluation of Aspergillus PCR protocols for testing serum specimens.Journal of clinical microbiology · 2011Article
- Ribosomal and mitochondrial DNA target for real-time PCR diagnosis of invasive aspergillosis.Journal of clinical microbiology · 2011Article
- Advances and prospects for molecular diagnostics of fungal infections.Current infectious disease reports · 2010Article
- Critical stages of extracting DNA from Aspergillus fumigatus in whole-blood specimens.Journal of clinical microbiology · 2010Article
- Aspergillus PCR: one step closer to standardization.Journal of clinical microbiology · 2010Article
- Diagnosis of invasive aspergillosis using bronchoalveolar lavage in haematology patients: influence of bronchoalveolar lavage human DNA content on real-time PCR performance.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Multidisciplinary approach to the treatment of invasive fungal infections in adult patients. Prophylaxis, empirical, preemptive or targeted therapy, which is the best in the different hosts?Therapeutics and clinical risk management · 2008Article
- Results of a local antibiotic management program on antibiotic use in a tertiary intensive care unit in Hungary.Infection · 2008Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Positive galactomannan (GM) anti-genemias are included as a microbiological item in the diagnosis of probable or possible invasive aspergillosis (IA). Because false-positive GM results frequently occur, at least two positive results on two different samples are required. Waiting for clinical specimens can delay the initiation of treatment. As an alternative, we wondered whether detection of circulating Aspergillus DNA on the first positive GM serum sample could aid in diagnosing IA. Therefore, we retrospectively screened the first GM-positive serum samples from 29 patients from our hematology unit for Aspergillus DNA using real-time PCR. We compared the real-time PCR results with the final classification of proven, probable, and possible IA according to consensual criteria. No clear correlation between PCR results and the classification with the medical files could be shown. However, a positive PCR result was associated with a poor prognosis (Fisher's test; P=0.01). Our preliminary data suggest that a positive PCR result could indicate a more advanced stage of the disease. Therefore, concomitant positive PCR and GM results may justify the initiation of antifungal therapy in neutropenic patients. In contrast, a negative PCR on the first positive GM sample may argue for postponing costly antifungal administration until additional arguments for the diagnosis of IA are presented.
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