ArticleJournal of clinical microbiology2005
Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed.
- Omics and Multiomics-Based Diagnostics for Invasive Candidiasis: Toward Precision Medicine.Molecular & cellular proteomics : MCP · 2025Review
- HTS and PCR Methods Are the Most Used in the Diagnosis of Aspergillosis: Advantages over Other Molecular Methods.Journal of fungi (Basel, Switzerland) · 2025Review
- Morphological and molecular identification of fungi isolated from spoilt apples in Ota metropolis.BMC microbiology · 2025Article
- Visible DNA microarray and loop-mediated isothermal amplification (LAMP) for the identification of Cryptococcus species recovered from culture medium and cerebrospinal fluid of patients with meningitis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2020Article
- Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.FEMS microbiology reviews · 2019Review
- Mycobiome diversity: high-throughput sequencing and identification of fungi.Nature reviews. Microbiology · 2019Review
- Visible DNA Microarray System as an Adjunctive Molecular Test in Identification of Pathogenic Fungi Directly from a Blood Culture Bottle.Journal of clinical microbiology · 2018Article
- Advances in Candida detection platforms for clinical and point-of-care applications.Critical reviews in biotechnology · 2017Review
- High Frequency of Chlamydia trachomatis Mixed Infections Detected by Microarray Assay in South American Samples.PloS one · 2016Article
- Solid and Suspension Microarrays for Microbial Diagnostics.Methods in microbiology · 2015Article
- [Differential diagnosis for detection of hyphae in tissue].Der Pathologe · 2013Review
- Visual analysis of DNA microarray data for accurate molecular identification of non-albicans Candida isolates from patients with candidemia episodes.Journal of clinical microbiology · 2013Article
- Molecular identification of isolated fungi from stored apples in Riyadh, Saudi Arabia.Saudi journal of biological sciences · 2013Article
- Oral candidosis in lichen planus: the diagnostic approach is of major therapeutic importance.Clinical oral investigations · 2013Article
- Accurate and rapid identification of Candida spp. frequently associated with fungemia by using PCR and the microarray-based Prove-it Sepsis assay.Journal of clinical microbiology · 2012Article
- Rapid detection and identification of Aspergillus from lower respiratory tract specimens by use of a combined probe-high-resolution melting analysis.Journal of clinical microbiology · 2012Article
- Systemic candidiasis in farm-reared red-legged partridges (Alectoris rufa) caused by Leucosporidium spp.BMC veterinary research · 2012Article
- Rapid identification of allergenic and pathogenic molds in environmental air by an oligonucleotide array.BMC infectious diseases · 2011Article
- Chromoblastomycosis after a leech bite complicated by myiasis: a case report.BMC infectious diseases · 2011Article
- Early identification of sepsis.Current infectious disease reports · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. Conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. We developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic Candida and Aspergillus species. Oligonucleotide probes were designed by exploiting the sequence variations of the internal transcribed spacer (ITS) regions of the rRNA gene cassette to identify Candida albicans, Candida dubliniensis, Candida krusei, Candida glabrata, Candida tropicalis, Candida parapsilosis, Candida guilliermondii, Candida lusitaniae, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, and Aspergillus terreus. By using universal fungal primers (ITS 1 and ITS 4) directed toward conserved regions of the 18S and 28S rRNA genes, respectively, the fungal ITS target regions could be simultaneously amplified and fluorescently labeled. To establish the system, 12 pre-characterized fungal strains were analyzed; and the method was validated by using 21 clinical isolates as blinded samples. As the microarray was able to detect and clearly identify the fungal pathogens within 4 h after DNA extraction, this system offers an interesting potential for clinical microbiology laboratories.
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