ArticleLangmuir : the ACS journal of surfaces and colloids2005
Lipid bilayers on polyacrylamide brushes for inclusion of membrane proteins.
Article in Langmuir : the ACS journal of surfaces and colloids, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Using the Water Absorption Ability of Dried Hydrogels to Form Hydrogel-Supported Lipid Bilayers.Gels (Basel, Switzerland) · 2023Article
- Effects of ethanol and n-butanol on the fluidity of supported lipid bilayers.Chemistry and physics of lipids · 2021Article
- Substrate-supported Model Membrane as a Versatile Analytical/Biosensing Platform.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Nanodomain Formation in Planar Supported Lipid Bilayers Composed of Fluid and Polymerized Dienoyl Lipids.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Fabrication of microstructured binary polymer brush "corrals" with integral pH sensing for studies of proton transport in model membrane systems.Chemical science · 2018Article
- Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers.The European physical journal. E, Soft matter · 2016Article
- Insertion and self-diffusion of a monotopic protein, the Aquifex aeolicus sulfide quinone reductase, in supported lipid bilayers.The European physical journal. E, Soft matter · 2015Article
- Comparison of [corrected] actin- and glass-supported phospholipid bilayer diffusion coefficients.Biophysical journal · 2015Article
- New poly(amino acid methacrylate) brush supports the formation of well-defined lipid membranes.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Phospholipid diffusion coefficients of cushioned model membranes determined via z-scan fluorescence correlation spectroscopy.Langmuir : the ACS journal of surfaces and colloids · 2013Article
- Coupling supported lipid bilayer electrophoresis with matrix-assisted laser desorption/ionization-mass spectrometry imaging.Analytical chemistry · 2013Article
- Protein-phospholipid interactions in nonclassical protein secretion: problem and methods of study.International journal of molecular sciences · 2013Article
- Beyond Saffman-Delbruck approximation: a new regime for 2D diffusion of α-hemolysin complexes in supported lipid bilayer.The European physical journal. E, Soft matter · 2012Article
- Supported bilayer electrophoresis under controlled buffer conditions.Analytical chemistry · 2011Article
- Noninvasive measurements of integrin microclustering under altered membrane cholesterol levels.Biophysical journal · 2010Article
- Field-effect detection using phospholipid membranes.Science and technology of advanced materials · 2010Review
- Double cushions preserve transmembrane protein mobility in supported bilayer systems.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- In-channel atom-transfer radical polymerization of thermoset polyester microfluidic devices for bioanalytical applications.Electrophoresis · 2007Article
- High-resolution AFM of membrane proteins directly incorporated at high density in planar lipid bilayer.Biophysical journal · 2006Article
- Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry.Analytical chemistry · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
The ability of neutral polymer cushions to support neutral lipid bilayers for the incorporation of mobile transmembrane proteins was investigated. Polyacrylamide brush layers were grown on fused silica using atom-transfer radical polymerization to provide polymer layers of 2.5-, 5- and 10-nm thickness. Lipid bilayers composed of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine) were formed by vesicle fusion onto bare fused silica and onto each of the polyacrylamide layers. Bilayer fluidity was assessed by the diffusion of a probe, NBD-labeled phosphatidylcholine, using fluorescence recovery after photobleaching. A transmembrane protein, the human delta-opioid receptor, was inserted into each lipid bilayer, and its ability to bind a synthetic ligand, DPDPE, cyclic[2-d-penicillamine, 5-d-penicillamine]enkephalin, was detected using single-molecule fluorescence spectroscopy by labeling this ligand with a rhodamine dye. The transmembrane protein was observed to bind the ligand for all bilayers tested. The protein's electrophoretic mobility was probed by monitoring the fluorescence from the bound ligand. The 5-nm polyacrylamide thickness gave the fastest diffusion for the fluorescent lipid probe (D(1) = 2.0(+/-1.2) x 10(-7) and D(2) = 1.2(+/-0.5) x 10(-6) cm(2)/s) and also the largest electrophoretic mobility for the transmembrane protein (3 x 10(-8) cm(2)/V.s). The optimum in polymer thickness is suggested to be a tradeoff between decoupling from the substrate and increasing roughness of the polymer surface.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.