ArticleApplied and environmental microbiology2005
Production of an engineered killer peptide in Nicotiana benthamiana by using a potato virus X expression system.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 40 citations in OpenAlex.
- Killer Peptide: an antibody-derived self-assembling peptide bridging antimicrobial and host-defense mechanisms.FEMS microbiology reviews · 2026Review
- Microbial Nanoparticles in Biological Plant Protection.International journal of molecular sciences · 2025Review
- Study on Antiviral Activity of Two Recombinant Antimicrobial Peptides Against Tobacco Mosaic Virus.Probiotics and antimicrobial proteins · 2019Article
- The Two-Faced Potato Virus X: From Plant Pathogen to Smart Nanoparticle.Frontiers in plant science · 2015Review
- Expression of synthetic human tropoelastin (hTE) protein in Nicotiana tabacum.GM crops & food · 2015Article
- Antibodies as an unlimited source of anti-infective, anti-tumour and immunomodulatory peptides.Science progress · 2014Review
- Antibody Peptide based antifungal immunotherapy.Frontiers in microbiology · 2012Article
- Plant heat shock protein 70 as carrier for immunization against a plant-expressed reporter antigen.Transgenic research · 2011Article
- Viral nanoparticles as macromolecular devices for new therapeutic and pharmaceutical approaches.International journal of physiology, pathophysiology and pharmacology · 2010Article
- Mycoviruses: future therapeutic agents of invasive fungal infections in humans?European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2010Review
- Viral vectors for production of recombinant proteins in plants.Journal of cellular physiology · 2008Review
- Use of a PTGS-MAR expression system for efficient in planta production of bioactive Arabidopsis thaliana plant defensins.Transgenic research · 2007Article
- Expression of a novel small antimicrobial protein from the seeds of motherwort (Leonurus japonicus) confers disease resistance in tobacco.Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The decapeptide killer peptide (KP) derived from the sequence of a single-chain, anti-idiotypic antibody acting as a functional internal image of a microbicidal, broad-spectrum yeast killer toxin (KT) was shown to exert a strong microbicidal activity against human pathogens. With the aim to exploit this peptide to confer resistance to plant pathogens, we assayed its antimicrobial activity against a broad spectrum of phytopathogenic bacteria and fungi. Synthetic KP exhibited antimicrobial activity in vitro towards Pseudomonas syringae, Erwinia carotovora, Botrytis cinerea, and Fusarium oxysporum. KP was also expressed in plants by using a Potato virus X (PVX)-derived vector as a fusion to the viral coat protein, yielding chimeric virus particles (CVPs) displaying the heterologous peptide. Purified CVPs showed enhanced antimicrobial activity against the above-mentioned plant pathogens and human pathogens such as Staphylococcus aureus and Candida albicans. Moreover, in vivo assays designed to challenge KP-expressing plants (as CVPs) with Pseudomonas syringae pv. tabaci showed enhanced resistance to bacterial attack. The results indicate that the PVX-based display system is a high-yield, rapid, and efficient method to produce and evaluate antimicrobial peptides in plants, representing a milestone for the large-scale production of high-added-value peptides through molecular farming. Moreover, KP is a promising molecule to be stably engineered in plants to confer broad-spectrum resistance to phytopathogens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.