ArticleApplied and environmental microbiology2005
Intracellular screen to identify metagenomic clones that induce or inhibit a quorum-sensing biosensor.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 199 citations in OpenAlex.
- Microbial Transcription Factor-Based Biosensors: Innovations from Design to Applications in Synthetic Biology.Biosensors · 2025Review
- Characterization of intestinal fungal communities through ITS amplicon from metagenomic libraries of sea cucumber (Microbiology resource announcements · 2025Article
- Analysis of metagenomic data.Nature reviews. Methods primers · 2025Article
- Cheating the Cheater: Suppressing False-Positive Enrichment during Biosensor-Guided Biocatalyst Engineering.ACS synthetic biology · 2022Article
- Recent Advances in the Mechanisms and Regulation of QS in Dairy Spoilage byFoods (Basel, Switzerland) · 2021Review
- Functional metagenomic analysis of quorum sensing signaling in a nitrifying community.NPJ biofilms and microbiomes · 2021Article
- Strategies for Natural Products Discovery from Uncultured Microorganisms.Molecules (Basel, Switzerland) · 2021Review
- Gene Sets and Mechanisms of Sulfate-Reducing Bacteria Biofilm Formation and Quorum Sensing With Impact on Corrosion.Frontiers in microbiology · 2021Review
- Improvements in Extraction Methods of High-molecular-weight DNA from Soils by Modifying Cell Lysis Conditions and Reducing Adsorption of DNA onto Soil Particles.Microbes and environments · 2021Article
- Effect of Quorum Sensing on the Ability ofApplied and environmental microbiology · 2019Article
- Biochemical Characteristics of Microbial Enzymes and Their Significance from Industrial Perspectives.Molecular biotechnology · 2019Review
- A neural network model predicts community-level signaling states in a diverse microbial community.PLoS computational biology · 2019Article
- Saline Environments as a Source of Potential Quorum Sensing Disruptors to Control Bacterial Infections: A Review.Marine drugs · 2019Review
- Insight Into the Microbial Co-occurrence and Diversity of 73 Grapevine (Frontiers in microbiology · 2019Article
- The evolving interface between synthetic biology and functional metagenomics.Nature chemical biology · 2018Review
- Concepts and Methods to Access Novel Antibiotics from Actinomycetes.Antibiotics (Basel, Switzerland) · 2018Review
- Mining Novel Constitutive Promoter Elements in Soil Metagenomic Libraries inFrontiers in microbiology · 2018Article
- Using natural products for drug discovery: the impact of the genomics era.Expert opinion on drug discovery · 2017Review
- HqiA, a novel quorum-quenching enzyme which expands the AHL lactonase family.Scientific reports · 2017Article
- Quorum Sensing and the Use of Quorum Quenchers as Natural Biocides to Inhibit Sulfate-Reducing Bacteria.Antibiotics (Basel, Switzerland) · 2016Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The goal of this study was to design and evaluate a rapid screen to identify metagenomic clones that produce biologically active small molecules. We built metagenomic libraries with DNA from soil on the floodplain of the Tanana River in Alaska. We extracted DNA directly from the soil and cloned it into fosmid and bacterial artificial chromosome vectors, constructing eight metagenomic libraries that contain 53,000 clones with inserts ranging from 1 to 190 kb. To identify clones of interest, we designed a high throughput "intracellular" screen, designated METREX, in which metagenomic DNA is in a host cell containing a biosensor for compounds that induce bacterial quorum sensing. If the metagenomic clone produces a quorum-sensing inducer, the cell produces green fluorescent protein (GFP) and can be identified by fluorescence microscopy or captured by fluorescence-activated cell sorting. Our initial screen identified 11 clones that induce and two that inhibit expression of GFP. The intracellular screen detected quorum-sensing inducers among metagenomic clones that a traditional overlay screen would not. One inducing clone carries a LuxI homologue that directs the synthesis of an N-acyl homoserine lactone quorum-sensing signal molecule. The LuxI homologue has 62% amino acid sequence identity to its closest match in GenBank, AmfI from Pseudomonas fluorescens, and is on a 78-kb insert that contains 67 open reading frames. Another inducing clone carries a gene with homology to homocitrate synthase. Our results demonstrate the power of an intracellular screen to identify functionally active clones and biologically active small molecules in metagenomic libraries.
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