ArticleApplied and environmental microbiology2005
Genome-directed isolation of the key nitrogen fixer Leptospirillum ferrodiazotrophum sp. nov. from an acidophilic microbial community.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
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- Characterization and Optimization of Cellulose-Degrading Bacteria Isolated from Fecal Samples ofMicroorganisms · 2025Article
- Discovery and cultivation of prokaryotic taxa in the age of metagenomics and artificial intelligence.The ISME journal · 2025Article
- Review
- Meta-omics assisted microbial gene and strain resources mining in contaminant environment.Engineering in life sciences · 2024Review
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- Draft genome sequence of two "Candidatus Intestinicoccus colisanans" strains isolated from faeces of healthy humans.BMC research notes · 2023Article
- Isolation and Cultivation of Human Gut Microorganisms: A Review.Microorganisms · 2023Review
- Shedding light on the composition of extreme microbial dark matter: alternative approaches for culturing extremophiles.Frontiers in microbiology · 2023Review
- Opportunities and challenges of using metagenomic data to bring uncultured microbes into cultivation.Microbiome · 2022Article
- Strategies to access biosynthetic novelty in bacterial genomes for drug discovery.Nature reviews. Drug discovery · 2022Review
- LC-MS Based Metabolomics Analysis of Potato (Metabolites · 2022Article
- Editorial: Extremophiles: Microbial genomics and taxogenomics.Frontiers in microbiology · 2022Article
- Prokaryotic communities in the historic silver mine Reiche Zeche.Extremophiles : life under extreme conditions · 2021Article
- Genomic Insights into the Ecological Role and Evolution of a NovelApplied and environmental microbiology · 2021Article
- Metagenome-Assembled Genomes of Novel Taxa from an Acid Mine Drainage Environment.Applied and environmental microbiology · 2021Article
- Molecular Microbial Community Analysis as an Analysis Tool for Optimal Biogas Production.Microorganisms · 2021Review
22 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Analysis of assembled random shotgun sequence data from a low-diversity, subsurface acid mine drainage (AMD) biofilm revealed a single nif operon. This was found on a genome fragment belonging to a member of Leptospirillum group III, a lineage in the Nitrospirae phylum with no cultivated representatives. Based on the prediction that this organism is solely responsible for nitrogen fixation in the community, we pursued a selective isolation strategy to obtain the organism in pure culture. An AMD biofilm sample naturally abundant in Leptospirillum group III cells was homogenized, filtered, and serially diluted into a nitrogen-free liquid medium. The resulting culture in the terminal dilution grew autotrophically to a maximum cell density of approximately 10(6) cells/ml, oxidizing ferrous iron as the sole energy source. 16S rRNA-internal transcribed spacer region clone library analysis confirmed that the isolate is a member of Leptospirillum group III and that the culture is axenic. We propose the name Leptospirillum ferrodiazotrophum sp. nov. for this iron-oxidizing, free-living diazotroph. This study highlights how environmental sequence data can provide insights for culturing previously uncultured microorganisms.
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