ArticleApplied and environmental microbiology2005
Phylum- and class-specific PCR primers for general microbial community analysis.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 132 citations in OpenAlex.
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- Identification of Cartilage Microbial DNA Signatures and Associations With Knee and Hip Osteoarthritis.Arthritis & rheumatology (Hoboken, N.J.) · 2020Article
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- Employment of Near Full-Length Ribosome Gene TA-Cloning and Primer-Blast to Detect Multiple Species in a Natural Complex Microbial Community Using Species-Specific Primers Designed with Their Genome Sequences.Molecular biotechnology · 2016Article
- Water level changes affect carbon turnover and microbial community composition in lake sediments.FEMS microbiology ecology · 2016Article
- Colonization of Hospital Water Networks by Gemmata massiliana, a New Planctomycetes Bacterium.Current microbiology · 2015Article
- Phytobeneficial Properties of Bacteria Isolated from the Rhizosphere of Maize in Southwestern Nigerian Soils.Applied and environmental microbiology · 2015Article
- Microencapsulated Bifidobacterium longum subsp. infantis ATCC 15697 favorably modulates gut microbiota and reduces circulating endotoxins in F344 rats.BioMed research international · 2014Article
- Effects of bacterial inoculants on the indigenous microbiome and secondary metabolites of chamomile plants.Frontiers in microbiology · 2014Article
- Denitrification in agriculturally impacted streams: seasonal changes in structure and function of the bacterial community.PloS one · 2014Article
- Impacts of labile organic carbon concentration on organic and inorganic nitrogen utilization by a stream biofilm bacterial community.Applied and environmental microbiology · 2013Article
- Biostimulation of the autochthonous microbial community for the depletion of polychlorinated biphenyls (PCBs) in contaminated sediments.Environmental science and pollution research international · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amplification of a particular DNA fragment from a mixture of organisms by PCR is a common first step in methods of examining microbial community structure. The use of group-specific primers in community DNA profiling applications can provide enhanced sensitivity and phylogenetic detail compared to domain-specific primers. Other uses for group-specific primers include quantitative PCR and library screening. The purpose of the present study was to develop several primer sets targeting commonly occurring and important groups. Primers specific for the 16S ribosomal sequences of Alphaproteobacteria, Betaproteobacteria, Bacilli, Actinobacteria, and Planctomycetes and for parts of both the 18S ribosomal sequence and the internal transcribed spacer region of Basidiomycota were examined. Primers were tested by comparison to sequences in the ARB 2003 database, and chosen primers were further tested by cloning and sequencing from soil community DNA. Eighty-five to 100% of the sequences obtained from clone libraries were found to be placed with the groups intended as targets, demonstrating the specificity of the primers under field conditions. It will be important to reevaluate primers over time because of the continual growth of sequence databases and revision of microbial taxonomy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.