ArticleApplied and environmental microbiology2005
Investigation of specific substitutions in virulence genes characterizing phenotypic groups of low-virulence field strains of Listeria monocytogenes.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 96 citations in OpenAlex.
- Biofilm forming capacity, sanitizer tolerance, and genetic characterization of persistent and non-persistentBiofilm · 2026Article
- Large-scale phenotyping and comparative genomics reveal genetic features of Listeria persistence in epithelial cells.PLoS pathogens · 2026Article
- The immune response modulated by inoculation of commensal bacteria at birth impacts the gut microbiota and preventsGut microbes · 2025Article
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- Human Listeriosis.Clinical microbiology reviews · 2023Review
- Assessment of the influence of selected stress factors on the growth and survival of Listeria monocytogenes.BMC microbiology · 2023Article
- Deletion ofFrontiers in microbiology · 2023Article
- Characterization of Listeria monocytogenes isolated from wildlife in central New York.Veterinary medicine and science · 2022Article
- NonhemolyticMicroorganisms · 2022Review
- Involvement of a putative ATP-Binding Cassette (ABC) Involved in manganese transport in virulence of Listeria monocytogenes.PloS one · 2022Article
- Bacteriophage-ResistantViruses · 2021Article
- Effect of Temperatures Used in Food Storage on Duration of Heat Stress Induced Invasiveness ofMicroorganisms · 2019Article
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- Virulence Gene Sequencing Highlights Similarities and Differences in Sequences inFrontiers in microbiology · 2018Article
- Article
- Comparative genomics of human and non-human Listeria monocytogenes sequence type 121 strains.PloS one · 2017Article
- Listeria monocytogenes sequence type 1 is predominant in ruminant rhombencephalitis.Scientific reports · 2016Article
- Tolerance of Listeria monocytogenes to Quaternary Ammonium Sanitizers Is Mediated by a Novel Efflux Pump Encoded by emrE.Applied and environmental microbiology · 2016Article
- PrfA regulation offsets the cost of Listeria virulence outside the host.Environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several models have shown that virulence varies from one strain of Listeria monocytogenes to another, but little is known about the cause of low virulence. Twenty-six field L. monocytogenes strains were shown to be of low virulence in a plaque-forming assay and in a subcutaneous inoculation test in mice. Using the results of cell infection assays and phospholipase activities, the low-virulence strains were assigned to one of four groups by cluster analysis and then virulence-related genes were sequenced. Group I included 11 strains that did not enter cells and had no phospholipase activity. These strains exhibited a mutated PrfA; eight strains had a single amino acid substitution, PrfAK220T, and the other three had a truncated PrfA, PrfADelta174-237. These genetic modifications could explain the low virulence of group I strains, since mutated PrfA proteins were inactive. Group II and III strains entered cells but did not form plaques. Group II strains had low phosphatidylcholine phospholipase C activity, whereas group III strains had low phosphatidylinositol phospholipase C activity. Several substitutions were observed for five out of six group III strains in the plcA gene and for one out of three group II strains in the plcB gene. Group IV strains poorly colonized spleens of mice and were practically indistinguishable from fully virulent strains on the basis of the above-mentioned in vitro criteria. These results demonstrate a relationship between the phenotypic classification and the genotypic modifications for at least group I and III strains and suggest a common evolution of these strains within a group.
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