ArticleApplied and environmental microbiology2005
Specific detection, isolation, and characterization of selected, previously uncultured members of the freshwater bacterioplankton community.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 97 citations in OpenAlex.
- A guide to the natural history of freshwater lake bacteria.Microbiology and molecular biology reviews : MMBR · 2011Pooled it
- A Novel Species Description of Sphingobium agrisoil sp. nov.Current microbiology · 2026Article
- Microbial model communities exhibit widespread metabolic interdependencies.Communications biology · 2025Article
- Bringing the uncultivated microbial majority of freshwater ecosystems into culture.Nature communications · 2025Article
- Growth of microalgae and cyanobacteria consortium in a photobioreactor treating liquid anaerobic digestate from vegetable waste.Scientific reports · 2023Article
- A Preliminary Study of Bacterioplankton Community Structure in the Taiyangshan Wetland in Ningxia and Its Driving Factors.International journal of environmental research and public health · 2022Article
- Spatial insurance in multi-trophic metacommunities.Ecology letters · 2019Article
- Discovery pipelines for marine resources: an ocean of opportunity for biotechnology?World journal of microbiology & biotechnology · 2019Review
- The dormant blood microbiome in chronic, inflammatory diseases.FEMS microbiology reviews · 2015Review
- Pyrosequencing analysis of roof-harvested rainwater and river water used for domestic purposes in Luthengele village in the Eastern Cape Province of South Africa.Environmental monitoring and assessment · 2015Article
- Article
- Are uncultivated bacteria really uncultivable?Microbes and environments · 2012Review
- Identification and targeted cultivation of abundant novel freshwater sphingomonads and analysis of their population substructure.Applied and environmental microbiology · 2011Article
- Thalassospira sp. isolated from the oligotrophic eastern Mediterranean Sea exhibits chemotaxis toward inorganic phosphate during starvation.Applied and environmental microbiology · 2011Article
- Influence of light and anoxia on chemiosmotic energy conservation in Dinoroseobacter shibae.Environmental microbiology reports · 2011Article
- Do patterns of bacterial diversity along salinity gradients differ from those observed for macroorganisms?PloS one · 2011Article
- Scent of danger: floc formation by a freshwater bacterium is induced by supernatants from a predator-prey coculture.Applied and environmental microbiology · 2010Article
- Substrate utilization profiles of bacterial strains in plankton from the River Warnow, a humic and eutrophic river in north Germany.Microbial ecology · 2010Article
- Low taxon richness of bacterioplankton in high-altitude lakes of the eastern tibetan plateau, with a predominance of Bacteroidetes and Synechococcus spp.Applied and environmental microbiology · 2009Article
- Involvement of cell surface structures in size-independent grazing resistance of freshwater Actinobacteria.Applied and environmental microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput cultivation was combined with rapid and group-specific phylogenetic fingerprinting in order to recover representatives of three freshwater bacterioplankton communities. A total of 570 bacterial cultures were obtained by employing the most probable number and MicroDrop techniques. The majority of the cultured bacteria were closely related to previously uncultured bacteria and grouped with the alpha-Proteobacteria, beta-Proteobacteria, Actinobacteria, Firmicutes, or Flavobacteria-Cytophaga lineage. Correspondingly, the natural bacterioplankton community was analyzed by high-resolution phylogenetic fingerprinting of these five bacterial lineages. 16S rRNA gene fragments were generated for each lineage and subsequently separated by denaturing gradient gel electrophoresis. By the combination of five group-specific PCR protocols, the total number of 16S rRNA gene fingerprints generated from the natural communities was increased sixfold compared to conventional (eubacterial) fingerprinting. Four of the environmental alpha-Proteobacteria 16S rRNA gene sequences obtained from the natural community were found to be identical to those of bacterial isolates. One of these phylotypes was detected in 14 different cultures and hence represented the most frequently cultured bacterium. Three of these 14 strains were characterized in detail. Their complete 16S rRNA gene sequences showed only 93% similarity to that of Sandaracinobacter sibiricus, the closest relative described so far. The novel phylotype of bacterium is a strict aerobe capable of using numerous organic carbon substrates and contains bacteriochlorophyll a bound to two different photosynthetic light-harvesting complexes. Dot blot hybridization revealed that the strains occur in lakes of different trophic status and constitute up to 2% of the microbial community.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.