ArticleApplied and environmental microbiology2005
Development of a functional genomics platform for Sinorhizobium meliloti: construction of an ORFeome.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- A MoClo-Compatible Toolbox of ECF Sigma Factor-Based Regulatory Switches for Proteobacterial Chassis.Biodesign research · 2024Article
- The transport of mannitol inMicrobiology (Reading, England) · 2023Article
- Inability to Catabolize Rhamnose byMicroorganisms · 2022Article
- Galactitol catabolism in Sinorhizobium meliloti is dependent on a chromosomally encoded sorbitol dehydrogenase and a pSymB-encoded operon necessary for tagatose catabolism.Molecular genetics and genomics : MGG · 2019Article
- Characterization of Mutations That Affect the Nonoxidative Pentose Phosphate Pathway in Sinorhizobium meliloti.Journal of bacteriology · 2018Article
- LDSS-P: an advanced algorithm to extract functional short motifs associated with coordinated gene expression.Nucleic acids research · 2016Article
- The Sugar Kinase That Is Necessary for the Catabolism of Rhamnose in Rhizobium leguminosarum Directly Interacts with the ABC Transporter Necessary for Rhamnose Transport.Journal of bacteriology · 2015Article
- The Sinorhizobium meliloti sensor histidine kinase CbrA contributes to free-living cell cycle regulation.Microbiology (Reading, England) · 2013Article
- Directed construction and analysis of a Sinorhizobium meliloti pSymA deletion mutant library.Applied and environmental microbiology · 2013Article
- Characterization of the twin-arginine transport secretome in Sinorhizobium meliloti and evidence for host-dependent phenotypes.Applied and environmental microbiology · 2012Article
- A MultiSite Gateway™ vector set for the functional analysis of genes in the model Saccharomyces cerevisiae.BMC molecular biology · 2012Article
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- ViralORFeome: an integrated database to generate a versatile collection of viral ORFs.Nucleic acids research · 2010Article
- High-throughput screening of bacterial protein localization.Methods in enzymology · 2010Article
- Deletion of citrate synthase restores growth of Sinorhizobium meliloti 1021 aconitase mutants.Journal of bacteriology · 2009Article
- A portal for rhizobial genomes: RhizoGATE integrates a Sinorhizobium meliloti genome annotation update with postgenome data.Journal of biotechnology · 2009Article
- Regulatory and DNA repair genes contribute to the desiccation resistance of Sinorhizobium meliloti Rm1021.Applied and environmental microbiology · 2009Article
- Construction and expression of sugar kinase transcriptional gene fusions by using the Sinorhizobium meliloti ORFeome.Applied and environmental microbiology · 2008Article
- Formate-dependent autotrophic growth in Sinorhizobium meliloti.Journal of bacteriology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nitrogen-fixing, symbiotic bacterium Sinorhizobium meliloti reduces molecular dinitrogen to ammonia in a specific symbiotic context, supporting the nitrogen requirements of various forage legumes, including alfalfa. Determining the DNA sequence of the S. meliloti genome was an important step in plant-microbe interaction research, adding to the considerable information already available about this bacterium by suggesting possible functions for many of the >6,200 annotated open reading frames (ORFs). However, the predictive power of bioinformatic analysis is limited, and putting the role of these genes into a biological context will require more definitive functional approaches. We present here a strategy for genetic analysis of S. meliloti on a genomic scale and report the successful implementation of the first step of this strategy by constructing a set of plasmids representing 100% of the 6,317 annotated ORFs cloned into a mobilizable plasmid by using efficient PCR and recombination protocols. By using integrase recombination to insert these ORFs into other plasmids in vitro or in vivo (B. L. House et al., Appl. Environ. Microbiol. 70:2806-2815, 2004), this ORFeome can be used to generate various specialized genetic materials for functional analysis of S. meliloti, such as operon fusions, mutants, and protein expression plasmids. The strategy can be generalized to many other genome projects, and the S. meliloti clones should be useful for investigators wanting an accessible source of cloned genes encoding specific enzymes.
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