ArticleApplied and environmental microbiology2005
Use of 16S rRNA gene terminal restriction fragment analysis to assess the impact of solids retention time on the bacterial diversity of activated sludge.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Understanding the dynamics of a microbial consortium prior to and post the introduction of specific antiretroviral compounds in a moving bed biofilm reactor system.Water science and technology : a journal of the International Association on Water Pollution Research · 2025Article
- Time Series Genome-Centric Analysis Unveils Bacterial Response to Operational Disturbance in Activated Sludge.mSystems · 2019Article
- Volatile fatty acid augmentation and microbial community responses in anaerobic co-fermentation process of waste-activated sludge mixed with corn stalk and livestock manure.Environmental science and pollution research international · 2018Article
- Environmental yeast communities in vineyards in the mountains of Santa Catarina State, Brazil.World journal of microbiology & biotechnology · 2017Article
- Diel-scale temporal dynamics recorded for bacterial groups in Namib Desert soil.Scientific reports · 2017Article
- Bacterial community assembly in activated sludge: mapping beta diversity across environmental variables.MicrobiologyOpen · 2016Article
- Tracing the limits of organic micropollutant removal in biological wastewater treatment.Water research · 2016Article
- Disturbance and temporal partitioning of the activated sludge metacommunity.The ISME journal · 2015Article
- Article
- Assessing bacterial diversity in a seawater-processing wastewater treatment plant by 454-pyrosequencing of the 16S rRNA and amoA genes.Microbial biotechnology · 2013Article
- Microbial community composition of polyhydroxyalkanoate-accumulating organisms in full-scale wastewater treatment plants operated in fully aerobic mode.Microbes and environments · 2013Article
- Article
- Microwave radiation and reactor design influence microbial communities during methane fermentation.Journal of industrial microbiology & biotechnology · 2012Article
- Article
- Molecular characterization of activated sludge from a seawater-processing wastewater treatment plant.Microbial biotechnology · 2011Article
- Repetitive extragenic palindromic PCR (REP-PCR) as a method used for bulking process detection in activated sludge.Environmental monitoring and assessment · 2011Article
- Diversity of dominant bacterial taxa in activated sludge promotes functional resistance following toxic shock loading.Microbial ecology · 2011Article
- Balance of neutral and deterministic components in the dynamics of activated sludge floc assembly.Microbial ecology · 2011Article
- Nonrandom assembly of bacterial populations in activated sludge flocs.Microbial ecology · 2010Article
- Molecular identification of fecal pollution sources in water supplies by host-specific fecal DNA markers and Terminal Restriction Fragment Length Polymorphism profiles of 16S rRNA gene.Journal of microbiology (Seoul, Korea) · 2008Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes was used to investigate the reproducibility and stability in the bacterial community structure of laboratory-scale sequencing batch bioreactors (SBR) and to assess the impact of solids retention time (SRT) on bacterial diversity. Two experiments were performed. In each experiment two sets of replicate SBRs were operated for a periods of three times the SRT. One set was operated at an SRT of 2 days and another set was operated at an SRT of 8 days. Samples for T-RFLP analysis were collected from the two sets of replicate reactors. HhaI, MspI, and RsaI T-RFLP profiles were analyzed using cluster analysis and diversity statistics. Cluster analysis with Ward's method using Jaccard distance and Hellinger distance showed that the bacterial community structure in both sets of reactors from both experimental runs was dynamic and that replicate reactors were clustered together and evolved similarly from startup. Richness (S), evenness (E), the Shannon-Weaver index (H), and the reciprocal of Simpson's index (1/D) were calculated, and the values were compared between the two sets of reactors. Evenness values were higher for reactors operated at an SRT of 2 days. Statistically significant differences in diversity (H and D) between the two sets of reactors were tested using a randomization procedure, and the results showed that reactors from both experimental runs that were operated at an SRT of 2 days had higher diversity (H and D) at the 5% level. T-RFLP analysis with diversity indices proved to be a powerful tool to analyze changes in the bacterial community diversity in response to changes in the operational parameters of activated-sludge systems.
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