ArticleNucleic acids research2005
Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Analysis of RAS protein interactions in living cells reveals a mechanism for pan-RAS depletion by membrane-targeted RAS binders.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics.Biophysical journal · 2020Article
- Bi-allelic Homology-Directed Repair with Helper-Dependent Adenoviruses.Molecular therapy. Methods & clinical development · 2019Article
- DIRAS3 (ARHI) Blocks RAS/MAPK Signaling by Binding Directly to RAS and Disrupting RAS Clusters.Cell reports · 2019Article
- A massively parallel reporter assay dissects the influence of chromatin structure on cis-regulatory activity.Nature biotechnology · 2018Article
- Neoantigen Expression in Steady-State Langerhans Cells Induces CTL Tolerance.Journal of immunology (Baltimore, Md. : 1950) · 2017Article
- Inhibition of MARCH5 ubiquitin ligase abrogates MCL1-dependent resistance to BH3 mimetics via NOXA.Oncotarget · 2016Article
- Gene stacking by recombinases.Plant biotechnology journal · 2016Review
- Direct and Propagated Effects of Small Molecules on Protein-Protein Interaction Networks.Frontiers in bioengineering and biotechnology · 2015Review
- A versatile platform to analyze low-affinity and transient protein-protein interactions in living cells in real time.Cell reports · 2014Article
- SHP2E76K mutant promotes lung tumorigenesis in transgenic mice.Carcinogenesis · 2014Article
- Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT).Transgenic research · 2013Article
- Use of a molecular genetic platform technology to produce human Wnt proteins reveals distinct local and distal signaling abilities.PloS one · 2013Article
- Highly efficient site-specific transgenesis in cancer cell lines.Molecular cancer · 2012Article
- Functional analysis and consequences of Mdm2 E3 ligase inhibition in human tumor cells.Oncogene · 2012Article
- Target gene analysis by microarrays and chromatin immunoprecipitation identifies HEY proteins as highly redundant bHLH repressors.PLoS genetics · 2012Article
- Overlapping activation-induced cytidine deaminase hotspot motifs in Ig class-switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells.Nucleic acids research · 2011Article
- Article
- Inducible expression of coding and inhibitory RNAs from retargetable genomic loci.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Comparative analysis of mutants using transfection is complicated by clones exhibiting variable levels of gene expression due to copy number differences and genomic position effects. Recombinase-mediated cassette exchange (RMCE) can overcome these problems by introducing the target gene into pre-determined chromosomal loci, but recombination between the available recombinase targeting sites can reduce the efficiency of targeted integration. We developed a new LoxP site (designated L3), which when used with the original LoxP site (designated L2), allows highly efficient and directional replacement of chromosomal DNA with incoming DNA. A total of six independent LoxP integration sites introduced either by homologous recombination or retroviral delivery were analyzed; 70-80% of the clones analyzed in hamster and human cells were correct recombinants. We combined the RMCE strategy with a new, tightly regulated tetracycline induction system to produce a robust, highly reliable system for inducible transgene expression. We observed stable inducible expression for over 1 month, with uniform expression in the cell population and between clones derived from the same integration site. This system described should find significant applications for studies requiring high level and regulated transgene expression and for determining the effects of various stresses or oncogenic conditions in vivo and in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.