ArticleGenes & development2005
Response of RNA polymerase to ppGpp: requirement for the omega subunit and relief of this requirement by DksA.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 101 citations in OpenAlex.
- ppGpp regulates transcription elongation via direct and indirect inputs to RNA polymerase pausing and nucleotide addition.bioRxiv : the preprint server for biology · 2026Article
- Omega stabilizes RNA polymerase condensates and contributes to cellular fitness during acid stress.bioRxiv : the preprint server for biology · 2026Article
- The displacement of the σ70 finger in initial transcription is highly heterogeneous and promoter-dependent.Nucleic acids research · 2025Article
- A non-native C-terminal extension of the β' subunit compromises RNA polymerase and Rho functions.Molecular microbiology · 2022Article
- Control of a programmed cell death pathway in Pseudomonas aeruginosa by an antiterminator.Nature communications · 2021Article
- relA and spoT Gene Expression is Modulated in Salmonella Grown Under Static Magnetic Field.Current microbiology · 2021Article
- DksA-dependent regulation of RpoS contributes to Borrelia burgdorferi tick-borne transmission and mammalian infectivity.PLoS pathogens · 2021Article
- Transcriptome analysis reveals that the RNA polymerase-binding protein DksA1 has pleiotropic functions inThe Journal of biological chemistry · 2020Article
- The Stress-Responsive Alternative Sigma Factor SigB ofFrontiers in microbiology · 2020Review
- Guanosine Tetraphosphate Has a Similar Affinity for Each of Its Two Binding Sites onFrontiers in microbiology · 2020Article
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- Transcriptional Responses to ppGpp and DksA.Annual review of microbiology · 2018Review
- Allosteric Effector ppGpp Potentiates the Inhibition of Transcript Initiation by DksA.Molecular cell · 2018Article
- Insights on Osmotic Tolerance Mechanisms in Escherichia coli Gained from an rpoC Mutation.Bioengineering (Basel, Switzerland) · 2017Article
- Article
- Affinity Selection-Mass Spectrometry Identifies a Novel Antibacterial RNA Polymerase Inhibitor.ACS chemical biology · 2017Article
- Acclimation to High COPlant physiology · 2017Article
- ppGpp Binding to a Site at the RNAP-DksA Interface Accounts for Its Dramatic Effects on Transcription Initiation during the Stringent Response.Molecular cell · 2016Article
- Bacterial CRISPR: accomplishments and prospects.Current opinion in microbiology · 2015Review
- Recent functional insights into the role of (p)ppGpp in bacterial physiology.Nature reviews. Microbiology · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Previous studies have come to conflicting conclusions about the requirement for the omega subunit of RNA polymerase in bacterial transcription regulation. We demonstrate here that purified RNAP lacking omega does not respond in vitro to the effector of the stringent response, ppGpp. DksA, a transcription factor that works in concert with ppGpp to regulate rRNA expression in vivo and in vitro, fully rescues the ppGpp-unresponsiveness of RNAP lacking omega, likely explaining why strains lacking omega display a stringent response in vivo. These results demonstrate that omega plays a role in RNAP function (in addition to its previously reported role in RNAP assembly) and highlight the importance of inclusion of omega in RNAP purification protocols. Furthermore, these results suggest that either one or both of two short segments in the beta' subunit that physically link omega to the ppGpp-binding region of the enzyme may play crucial roles in ppGpp and DksA function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.