ArticleParticle and fibre toxicology2005
Ultrafine particles cause cytoskeletal dysfunctions in macrophages: role of intracellular calcium.
Article in Particle and fibre toxicology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 70 citations in OpenAlex.
- PM2.5 Synergizes WithFrontiers in pharmacology · 2022Article
- Role of indoor aerosols for COVID-19 viral transmission: a review.Environmental chemistry letters · 2021Review
- Developmental toxicity of carbon nanoparticles during embryogenesis in chicken.Environmental science and pollution research international · 2020Review
- Ultrafine Particulate Matter Increases Cardiac Ischemia/Reperfusion Injury via Mitochondrial Permeability Transition Pore.Cardiovascular toxicology · 2017Article
- Human peripheral blood mononuclear cells (PBMCs) from smokers release higher levels of IL-1-like cytokines after exposure to combustion-generated ultrafine particles.Scientific reports · 2017Article
- Protein oxidation and degradation caused by particulate matter.Scientific reports · 2016Article
- Effects of zinc oxide nanoparticles on Kupffer cell phagosomal motility, bacterial clearance, and liver function.International journal of nanomedicine · 2015Article
- Carbon black particle exhibits size dependent toxicity in human monocytes.International journal of inflammation · 2014Article
- The adverse effects of air pollution on the nervous system.Journal of toxicology · 2012Article
- Induction of inflammasome-dependent pyroptosis by carbon black nanoparticles.The Journal of biological chemistry · 2011Article
- Toxicity of Transition Metal Oxide Nanoparticles: Recent Insights from in vitro Studies.Materials (Basel, Switzerland) · 2010Review
- Ultrafine carbon particles down-regulate CYP1B1 expression in human monocytes.Particle and fibre toxicology · 2009Article
- Diesel exhaust particles activate the matrix-metalloproteinase-1 gene in human bronchial epithelia in a beta-arrestin-dependent manner via activation of RAS.Environmental health perspectives · 2009Article
- Biopharmaceutics and therapeutic potential of engineered nanomaterials.Current drug metabolism · 2008Review
- Cytokine responses of human lung cells (BEAS-2B) treated with micron-sized and nanoparticles of metal oxides compared to soil dusts.Particle and fibre toxicology · 2007Article
- Motion and twisting of magnetic particles ingested by alveolar macrophages in the human lung: effect of smoking and disease.Biomagnetic research and technology · 2006Article
- Effects of PM10 in human peripheral blood monocytes and J774 macrophages.Respiratory research · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundParticulate air pollution is reported to cause adverse health effects in susceptible individuals. Since most of these particles are derived form combustion processes, the primary composition product is carbon with a very small diameter (ultrafine, less than 100 nm in diameter). Besides the induction of reactive oxygen species and inflammation, ultrafine particles (UFP) can cause intracellular calcium transients and suppression of defense mechanisms of alveolar macrophages, such as impaired migration or phagocytosis.
methodsIn this study the role of intracellular calcium transients caused by UFP was studied on cytoskeleton related functions in J774A.1 macrophages. Different types of fine and ultrafine carbon black particles (CB and ufCB, respectively), such as elemental carbon (EC90), commercial carbon (Printex 90), diesel particulate matter (DEP) and urban dust (UD), were investigated. Phagosome transport mechanisms and mechanical cytoskeletal integrity were studied by cytomagnetometry and cell viability was studied by fluorescence microscopy. Macrophages were exposed in vitro with 100 and 320 microg UFP/ml/million cells for 4 hours in serum free medium. Calcium antagonists Verapamil, BAPTA-AM and W-7 were used to block calcium channels in the membrane, to chelate intracellular calcium or to inhibit the calmodulin signaling pathways, respectively.
resultsImpaired phagosome transport and increased cytoskeletal stiffness occurred at EC90 and P90 concentrations of 100 microg/ml/million cells and above, but not with DEP or UD. Verapamil and W-7, but not BAPTA-AM inhibited the cytoskeletal dysfunctions caused by EC90 or P90. Additionally the presence of 5% serum or 1% bovine serum albumin (BSA) suppressed the cytoskeletal dysfunctions. Cell viability showed similar results, where co-culture of ufCB together with Verapamil, W-7, FCS or BSA produced less cell dead compared to the particles only.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.