ArticleThe Biochemical journal2006
Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 72 citations in OpenAlex.
- SNF1/AMPK controls its own localization by phosphorylating its activating kinase Sak1.iScience · 2026Article
- 1,1-Diethoxyethane enhances aerobic respiration in human mitochondria via activation of AMP-activated protein kinase.Communications biology · 2026Article
- The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways.Frontiers in molecular biosciences · 2024Review
- Article
- Fine-Tuning of Energy Levels RegulatesFrontiers in physiology · 2020Article
- Increased mitochondrial respiration promotes survival from endoplasmic reticulum stress.Cell death and differentiation · 2019Article
- Multilayered regulation of TORC1-body formation in budding yeast.Molecular biology of the cell · 2019Article
- Substrate priming enhances phosphorylation by the budding yeast kinases Kin1 and Kin2.The Journal of biological chemistry · 2018Article
- Snf1 cooperates with the CWI MAPK pathway to mediate the degradation of Med13 following oxidative stress.Microbial cell (Graz, Austria) · 2018Article
- Amino acid metabolites that regulate G protein signaling during osmotic stress.PLoS genetics · 2017Article
- The yeast Hsp70 homolog Ssb: a chaperone for general de novo protein folding and a nanny for specific intrinsically disordered protein domains.Current genetics · 2017Review
- The β subunit of yeast AMP-activated protein kinase directs substrate specificity in response to alkaline stress.Cellular signalling · 2016Article
- The ubiquitin-conjugating enzyme, Ubc1, indirectly regulates SNF1 kinase activity via Forkhead-dependent transcription.Microbial cell (Graz, Austria) · 2016Article
- Activation and inhibition of Snf1 kinase activity by phosphorylation within the activation loop.Biochimica et biophysica acta · 2016Article
- Coordinate action of distinct sequence elements localizes checkpoint kinase Hsl1 to the septin collar at the bud neck in Saccharomyces cerevisiae.Molecular biology of the cell · 2016Article
- The Hsp70 homolog Ssb and the 14-3-3 protein Bmh1 jointly regulate transcription of glucose repressed genes in Saccharomyces cerevisiae.Nucleic acids research · 2016Article
- Article
- Cross-Talk between Carbon Metabolism and the DNA Damage Response in S. cerevisiae.Cell reports · 2015Article
- The SNF1 Kinase Ubiquitin-associated Domain Restrains Its Activation, Activity, and the Yeast Life Span.The Journal of biological chemistry · 2015Article
- Network reconstruction and validation of the Snf1/AMPK pathway in baker's yeast based on a comprehensive literature review.NPJ systems biology and applications · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Members of the Snf1/AMPK family of protein kinases are activated by distinct upstream kinases that phosphorylate a conserved threonine residue in the Snf1/AMPK activation loop. Recently, the identities of the Snf1- and AMPK-activating kinases have been determined. Here we describe the purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae. The identities of proteins associated with the Snf1-activating kinases were determined by peptide mass fingerprinting. These kinases, Sak1, Tos3 and Elm2 do not appear to require the presence of additional subunits for activity. Sak1 and Snf1 co-purify and co-elute in size exclusion chromatography, demonstrating that these two proteins form a stable complex. The Snf1-activating kinases phosphorylate the activation loop threonine of Snf1 in vitro with great specificity and are able to do so in the absence of beta and gamma subunits of the Snf1 heterotrimer. Finally, we showed that the Snf1 kinase domain isolated from bacteria as a GST fusion protein can be activated in vitro and shows substrate specificity in the absence of its beta and gamma subunits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.