ArticleThe Biochemical journal2006
Distinct roles for de novo versus hydrolytic pathways of sphingolipid biosynthesis in Saccharomyces cerevisiae.
Article in The Biochemical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- The dynamics and role of sphingolipids in eukaryotic organisms upon thermal adaptation.Progress in lipid research · 2020Review
- Sphingolipids are required for exocyst polarity and exocytic secretion inCell & bioscience · 2020Article
- Sphingolipids and mitochondrial function, lessons learned from yeast.Microbial cell (Graz, Austria) · 2014Review
- Primary cilia in stem cells and neural progenitors are regulated by neutral sphingomyelinase 2 and ceramide.Molecular biology of the cell · 2014Article
- Distinct signaling roles of ceramide species in yeast revealed through systematic perturbation and systems biology analyses.Science signaling · 2013Article
- Iron, glucose and intrinsic factors alter sphingolipid composition as yeast cells enter stationary phase.Biochimica et biophysica acta · 2013Article
- Article
- Sphingoid bases and the serine catabolic enzyme CHA1 define a novel feedforward/feedback mechanism in the response to serine availability.The Journal of biological chemistry · 2012Article
- Activation of the unfolded protein response pathway causes ceramide accumulation in yeast and INS-1E insulinoma cells.Journal of lipid research · 2012Article
- Modulation of mitochondrial outer membrane permeabilization and apoptosis by ceramide metabolism.PloS one · 2012Article
- Role for Sit4p-dependent mitochondrial dysfunction in mediating the shortened chronological lifespan and oxidative stress sensitivity of Isc1p-deficient cells.Molecular microbiology · 2011Article
- Sphingolipids mediate formation of mRNA processing bodies during the heat-stress response of Saccharomyces cerevisiae.The Biochemical journal · 2010Article
- The essential neutral sphingomyelinase is involved in the trafficking of the variant surface glycoprotein in the bloodstream form of Trypanosoma brucei.Molecular microbiology · 2010Article
- Degradation of host sphingomyelin is essential for Leishmania virulence.PLoS pathogens · 2009Article
- Defective CFTR increases synthesis and mass of sphingolipids that modulate membrane composition and lipid signaling.Journal of lipid research · 2009Article
- ISC1-dependent metabolic adaptation reveals an indispensable role for mitochondria in induction of nuclear genes during the diauxic shift in Saccharomyces cerevisiae.The Journal of biological chemistry · 2009Article
- Modulation of sphingolipid metabolism by the phosphatidylinositol-4-phosphate phosphatase Sac1p through regulation of phosphatidylinositol in Saccharomyces cerevisiae.The Journal of biological chemistry · 2009Article
- Classifying transcription factor targets and discovering relevant biological features.Biology direct · 2008Article
- Thematic review series: sphingolipids. New insights into sphingolipid metabolism and function in budding yeast.Journal of lipid research · 2008Review
- Thematic review series: sphingolipids. ISC1 (inositol phosphosphingolipid-phospholipase C), the yeast homologue of neutral sphingomyelinases.Journal of lipid research · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Saccharomyces cerevisiae produces the sphingolipid ceramide by de novo synthesis as well as by hydrolysis of complex sphingolipids by Isc1p (inositolphosphoceramide-phospholipase C), which is homologous with the mammalian neutral sphingomyelinases. Though the roles of sphingolipids in yeast stress responses are well characterized, it has been unclear whether Isc1p contributes to stress-induced sphingolipids. The present study was undertaken in order to distinguish the relative roles of de novo sphingolipid biosynthesis versus Isc1p-mediated sphingolipid production in the heat-stress response. Ceramide production was measured at normal and increased temperature in an ISC1 deletion and its parental strain (ISC1 being the gene that codes for Isc1p). The results showed that Isc1p contributes specifically to the formation of the C24-, C24:1- and C26-dihydroceramide species. The interaction between these two pathways of sphingolipid production was confirmed by the finding that ISC1 deletion is synthetically lethal with the lcb1-100 mutation. Interestingly, Isc1p did not contribute significantly to transient cell-cycle arrest or growth at elevated temperature, responses known to be regulated by the de novo pathway. In order to define specific contributions of ISC1, microarray hybridizations were performed, and analyses showed misregulation of genes involved in carbon source utilization and sexual reproduction, which was corroborated by defining a sporulation defect of the isc1Delta strain. These results indicate that the two pathways of ceramide production in yeast interact, but differ in their regulation of ceramides of distinct molecular species and serve distinct cellular functions.
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