ArticleMolecular and cellular biology1992
In vivo splicing of the beta tropomyosin pre-mRNA: a role for branch point and donor site competition.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Secondary structures in RNA synthesis, splicing and translation.Computational and structural biotechnology journal · 2022Review
- Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches.Nature reviews. Molecular cell biology · 2009Review
- The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit.Molecular and cellular biology · 2006Article
- Complex splicing pattern generates great diversity in human NF1 transcripts.BMC genomics · 2002Article
- Splicing enhancement in the yeast rp51b intron.RNA (New York, N.Y.) · 2000Article
- Polypyrimidine tract binding protein functions as a repressor to regulate alternative splicing of alpha-actinin mutally exclusive exons.Molecular and cellular biology · 1999Article
- Donor site competition is involved in the regulation of alternative splicing of the rat beta-tropomyosin pre-mRNA.RNA (New York, N.Y.) · 1999Article
- The polypyrimidine tract binding protein binds upstream of neural cell-specific c-src exon N1 to repress the splicing of the intron downstream.Molecular and cellular biology · 1997Article
- The SR splicing factors ASF/SF2 and SC35 have antagonistic effects on intronic enhancer-dependent splicing of the beta-tropomyosin alternative exon 6A.The EMBO journal · 1997Article
- Relative exon affinities and suboptimal splice site signals lead to non-equivalence of two cassette exons.Nucleic acids research · 1995Article
- A T to G mutation in the polypyrimidine tract of the second intron of the human beta-globin gene reduces in vitro splicing efficiency: evidence for an increased hnRNP C interaction.Nucleic acids research · 1995Article
- Smooth muscle-specific switching of alpha-tropomyosin mutually exclusive exon selection by specific inhibition of the strong default exon.The EMBO journal · 1994Article
- Alternative splicing of beta-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5'- and 3'-splice sites of the nonmuscle/smooth muscle exon 6.Nucleic acids research · 1994Article
- Promoter elements and transcriptional control of the chicken tropomyosin gene [corrected].Nucleic acids research · 1994Article
- A novel exon in the cystic fibrosis transmembrane conductance regulator gene activated by the nonsense mutation E92X in airway epithelial cells of patients with cystic fibrosis.The Journal of clinical investigation · 1994Article
- Several mRNAs with variable 3' untranslated regions and different stability encode the human PR264/SC35 splicing factor.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Control of calcitonin/calcitonin gene-related peptide pre-mRNA processing by constitutive intron and exon elements.Molecular and cellular biology · 1993Article
- Developmental analysis of tropomyosin gene expression in embryonic stem cells and mouse embryos.Molecular and cellular biology · 1993Article
- cis-acting sequences involved in exon selection in the chicken beta-tropomyosin gene.Molecular and cellular biology · 1992Article
- Intronic sequence with both negative and positive effects on the regulation of alternative transcripts of the chicken beta tropomyosin transcripts.Nucleic acids research · 1992Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chicken beta tropomyosin gene contains two sets of alternatively spliced, mutually exclusive exons whose utilization is developmentally regulated. Exons 6A and 6B are used in nonmuscle cells (or undifferentiated muscle cells) and skeletal muscle cells, respectively. A complex arrangement of cis-acting sequence elements is involved in alternative splicing regulation. We have performed an extensive mutational analysis on the sequence spanning the region from exon 6A to the constitutive exon 7. A large number of mutant minigenes have been tested in transfection assays of cultured myogenic cells, and the splicing products have been analyzed by cDNA polymerase chain reaction. We demonstrate that in undifferentiated myoblasts, exon 6B is skipped as a result of a negative control on its selection, while exon 6A is spliced as a default choice. We provide evidence that the focal point of such a regulation is localized in the intron upstream of exon 6B and probably involves the blockage of its associated branch point. In differentiated myotubes, in contrast, both exons are accessible to the splicing machinery. We show that the preferential choice of exon 6B in this splicing environment depends on the existence of a competition between the two exons for the flanking constitutive splice sites. We demonstrate that both the donors and the branch points of the two exons are involved in this competition.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.