ArticleMolecular and cellular biology1992
Differential binding of heterogeneous nuclear ribonucleoproteins to mRNA precursors prior to spliceosome assembly in vitro.
Article in Molecular and cellular biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
65 citing papers in PubMed, 142 citations in OpenAlex.
- The RNA binding complexes NF45-NF90 and NF45-NF110 associate dynamically with the c-fos gene and function as transcriptional coactivators.The Journal of biological chemistry · 2015Article
- Arginine methylation of hnRNP A2 does not directly govern its subcellular localization.PloS one · 2013Article
- Inhibition of human immunodeficiency virus replication by cell membrane-crossing oligomers.Molecular medicine (Cambridge, Mass.) · 2012Article
- Repression of prespliceosome complex formation at two distinct steps by Fox-1/Fox-2 proteins.Molecular and cellular biology · 2008Article
- Wnt signaling inside the nucleus.Cancer science · 2008Review
- Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145.Molecular and cellular biology · 2006Article
- Functional coupling of RNAP II transcription to spliceosome assembly.Genes & development · 2006Article
- A molecular link between SR protein dephosphorylation and mRNA export.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The amelogenin loci span an ancient pseudoautosomal boundary in diverse mammalian species.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- A proteomic approach to the identification of heterogeneous nuclear ribonucleoproteins as a new family of poly(ADP-ribose)-binding proteins.The Biochemical journal · 2003Article
- Association of the 72/74-kDa proteins, members of the heterogeneous nuclear ribonucleoprotein M group, with the pre-mRNA at early stages of spliceosome assembly.The Biochemical journal · 2002Article
- Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.RNA (New York, N.Y.) · 2002Article
- Herpes simplex virus ICP27 protein provides viral mRNAs with access to the cellular mRNA export pathway.The EMBO journal · 2001Article
- Assembly and transport of a premessenger RNP particle.Proceedings of the National Academy of Sciences of the United States of America · 2001Review
- Selection of alternative 5' splice sites: role of U1 snRNP and models for the antagonistic effects of SF2/ASF and hnRNP A1.Molecular and cellular biology · 2000Article
- Cooperative assembly of an hnRNP complex induced by a tissue-specific homolog of polypyrimidine tract binding protein.Molecular and cellular biology · 2000Article
- Isolation and characterization of polyadenylation complexes assembled in vitro.RNA (New York, N.Y.) · 2000Article
- Functional Cus1p is found with Hsh155p in a multiprotein splicing factor associated with U2 snRNA.Molecular and cellular biology · 2000Article
- Resolution of the mammalian E complex and the ATP-dependent spliceosomal complexes on native agarose mini-gels.RNA (New York, N.Y.) · 1999Article
- hnRNP H is a component of a splicing enhancer complex that activates a c-src alternative exon in neuronal cells.Molecular and cellular biology · 1999Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the composition of the earliest detectable complex (H) assembled on pre-mRNA during the in vitro splicing reaction. We show that most of the proteins in this complex correspond to heterogeneous nuclear ribonucleoproteins (hnRNP), a set of abundant RNA-binding proteins that bind nascent RNA polymerase II transcripts in vivo. Thus, these studies establish a direct parallel between the initial events of RNA processing in vitro and in vivo. In contrast to previous studies, in which total hnRNP particles were isolated from mammalian nuclei, we determined the hnRNP composition of complexes assembled on individual RNAs of defined sequence. We found that a unique combination of hnRNP proteins is associated with each RNA. Thus, our data provide direct evidence for transcript-dependent assembly of pre-mRNA in hnRNP complexes. The observation that pre-mRNA is differentially bound by hnRNP proteins prior to spliceosome assembly suggests the possibility that RNA packaging could play a central role in the mechanism of splice site selection, as well as other posttranscriptional events.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.