ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
Random sheared fosmid library as a new genomic tool to accelerate complete finishing of rice (Oryza sativa spp. Nipponbare) genome sequence: sequencing of gap-specific fosmid clones uncovers new euchromatic portions of the genome.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Article
- The AKR gene family and modifying sex ratios in palms through abiotic stress responsiveness.Functional & integrative genomics · 2015Article
- Characterization of alginate lyase gene using a metagenomic library constructed from the gut microflora of abalone.Journal of industrial microbiology & biotechnology · 2012Article
- Bacterial artificial chromosome libraries of pulse crops: characteristics and applications.Journal of biomedicine & biotechnology · 2012Review
- Advances in BAC-based physical mapping and map integration strategies in plants.Journal of biomedicine & biotechnology · 2012Review
- Current challenges in de novo plant genome sequencing and assembly.Genome biology · 2012Review
- The first insight into the Taxus genome via fosmid library construction and end sequencing.Molecular genetics and genomics : MGG · 2011Article
- Screening and characterization of a cellulase gene from the gut microflora of abalone using metagenomic library.Journal of microbiology (Seoul, Korea) · 2011Article
- Genome-wide BAC-end sequencing of Cucumis melo using two BAC libraries.BMC genomics · 2010Article
- Validation of rice genome sequence by optical mapping.BMC genomics · 2007Article
- An improved method to identify BAC clones using pooled overgos.Nucleic acids research · 2007Article
- Sequence comparison of distal and proximal ribosomal DNA arrays in rice (Oryza sativa L.) chromosome 9S and analysis of their flanking regions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The International Rice Genome Sequencing Project has recently announced the high-quality finished sequence that covers nearly 95% of the japonica rice genome representing 370 Mbp. Nevertheless, the current physical map of japonica rice contains 62 physical gaps corresponding to approximately 5% of the genome, that have not been identified/represented in the comprehensive array of publicly available BAC, PAC and other genomic library resources. Without finishing these gaps, it is impossible to identify the complete complement of genes encoded by rice genome and will also leave us ignorant of some 5% of the genome and its unknown functions. In this article, we report the construction and characterization of a tenfold redundant, 40 kbp insert fosmid library generated by random mechanical shearing. We demonstrated its utility in refining the physical map of rice by identifying and in silico mapping 22 gap-specific fosmid clones with particular emphasis on chromosomes 1, 2, 6, 7, 8, 9 and 10. Further sequencing of 12 of the gap-specific fosmid clones uncovered unique rice genome sequence that was not previously reported in the finished IRGSP sequence and emphasizes the need to complete finishing of the rice genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.