ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
Site-specific recombination in Zea mays.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Clean gene technology to develop selectable marker-free pod borer-resistant transgenic pigeon pea events involving the constitutive expression of Cry1Ac.Applied microbiology and biotechnology · 2022Article
- Precise Characterization and Tracking of Stably Inherited Artificial Minichromosomes Made by Telomere-Mediated Chromosome Truncation inFrontiers in plant science · 2021Article
- Site-specific recombinase genome engineering toolkit in maize.Plant direct · 2020Article
- An Efficient Gene Excision System in Maize.Frontiers in plant science · 2020Article
- Progress of targeted genome modification approaches in higher plants.Plant cell reports · 2016Review
- Selectable Marker Gene Removal and Expression of Transgene by Inducible Promoter Containing FFDDIranian journal of biotechnology · 2015Article
- Utilization of PVX-Cre expression vector in potato.Transgenic research · 2012Article
- Improved FLP recombinase, FLPe, efficiently removes marker gene from transgene locus developed by Cre-lox mediated site-specific gene integration in rice.Molecular biotechnology · 2011Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- Lysine racemase: a novel non-antibiotic selectable marker for plant transformation.Plant molecular biology · 2010Article
- ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome.Transgenic research · 2009Article
- Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.Plant cell reports · 2008Article
- Marker-free transgenic plants through genetically programmed auto-excision.Plant physiology · 2007Article
- Construction and behavior of engineered minichromosomes in maize.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Functionality of the beta/six site-specific recombination system in tobacco and Arabidopsis: a novel tool for genetic engineering of plant genomes.Plant molecular biology · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The elimination of marker genes after selection is recommended for the commercial use of genetically modified plants. We compared the applicability of the two site-specific recombination systems Cre/lox and Flp/FRT for marker gene elimination in maize plants. The selection marker gene pat surrounded by two identically directed lox or FRT sites was introduced into maize. Sexual crossing with plants harboring the corresponding constitutively expressed recombinase led to the precise and complete excision of the lox-flanked marker gene in the F1 progeny, whereas Flp-mediated recombination of FRT sequences occurred rarely. Further examination of site-specific integration was done by biolistic bombardment of immature embryos harboring only one lox site with a lox.uidA sequence with results indicating directed integration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.