ArticleMolecular and cellular biology2005
The condensin I subunit Barren/CAP-H is essential for the structural integrity of centromeric heterochromatin during mitosis.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 104 citations in OpenAlex.
- Cross-species incompatibilities offer new insights into the functional consequences of satellite DNA evolution.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2026Review
- Condensin I but not Condensin II is crucial for mitotic chromosome mechanics.Nature communications · 2026Article
- An electrostatic repulsion model of centromere organisation.bioRxiv : the preprint server for biology · 2025Article
- SRBD1 facilitates chromosome segregation by promoting topoisomerase IIα localization to mitotic chromosomes.Nature communications · 2025Article
- Review
- NCAPH Promotes the Proliferation of Prostate Cancer Cells Via Modulating the E2F1 Mediated PI3K/AKT/mTOR Axis.International journal of medical sciences · 2025Article
- Review
- Vertebrate centromere architecture: from chromatin threads to functional structures.Chromosoma · 2024Review
- Vertebrate centromeres in mitosis are functionally bipartite structures stabilized by cohesin.Cell · 2024Article
- Article
- Unveiling the Significance of NCAP Family Genes in Adrenocortical Carcinoma and Adenoma Pathogenesis: A Molecular Bioinformatics Exploration.Cancer informatics · 2024Article
- Genome control by SMC complexes.Nature reviews. Molecular cell biology · 2023Review
- The material properties of mitotic chromosomes.Current opinion in structural biology · 2023Review
- Mitotic chromosome condensation requires phosphorylation of the centromeric protein KNL-2 in C. elegans.Journal of cell science · 2021Article
- Functional Diversification of Chromatin on Rapid Evolutionary Timescales.Annual review of genetics · 2021Review
- The condensin holocomplex cycles dynamically between open and collapsed states.Nature structural & molecular biology · 2020Article
- Identification of NCAPH as a biomarker for prognosis of breast cancer.Molecular biology reports · 2020Article
- Condensin I is required for faithful meiosis in Drosophila males.Chromosoma · 2020Article
- Article
- Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During cell division, chromatin undergoes structural changes essential to ensure faithful segregation of the genome. Condensins, abundant components of mitotic chromosomes, are known to form two different complexes, condensins I and II. To further examine the role of condensin I in chromosome structure and in particular in centromere organization, we depleted from S2 cells the Drosophila CAP-H homologue Barren, a subunit exclusively associated with condensin I. In the absence of Barren/CAP-H the condensin core subunits DmSMC4/2 still associate with chromatin, while the other condensin I non-structural maintenance of chromosomes family proteins do not. Immunofluorescence and in vivo analysis of Barren/CAP-H-depleted cells showed that mitotic chromosomes are able to condense but fail to resolve sister chromatids. Additionally, Barren/CAP-H-depleted cells show chromosome congression defects that do not appear to be due to abnormal kinetochore-microtubule interaction. Instead, the centromeric and pericentromeric heterochromatin of Barren/CAP-H-depleted chromosomes shows structural problems. After bipolar attachment, the centromeric heterochromatin organized in the absence of Barren/CAP-H cannot withstand the forces exerted by the mitotic spindle and undergoes irreversible distortion. Taken together, our data suggest that the condensin I complex is required not only to promote sister chromatid resolution but also to maintain the structural integrity of centromeric heterochromatin during mitosis.
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