ArticleRNA (New York, N.Y.)2005
Estimating RNA editing efficiency of five editing sites in the serotonin 2C receptor by pyrosequencing.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
- Detection of Developmental Asexual Stage-Specific RNA Editing Events inMicroorganisms · 2024Article
- Abundant and selective RNA-editing events in the medicinal mushroom Ganoderma lucidum.Genetics · 2014Article
- A role of ADAR2 and RNA editing of glutamate receptors in mood disorders and schizophrenia.Molecular brain · 2014Article
- Cell type-specific gene expression and editing responses to chronic fluoxetine treatment in the in vivo mouse brain and their relevance for stress-induced anhedonia.Neurochemical research · 2012Article
- Quantification of adenosine-to-inosine editing of microRNAs using a conventional method.Nature protocols · 2012Article
- Quantitative analysis of 5HT(2C) receptor RNA editing patterns in psychiatric disorders.Neurobiology of disease · 2012Review
- Fluoxetine affects GluK2 editing, glutamate-evoked Ca(2+) influx and extracellular signal-regulated kinase phosphorylation in mouse astrocytes.Journal of psychiatry & neuroscience : JPN · 2011Article
- Altered ADAR 2 equilibrium and 5HT(2C) R editing in the prefrontal cortex of ADAR 2 transgenic mice.Genes, brain, and behavior · 2011Article
- Assessing serotonin receptor mRNA editing frequency by a novel ultra high-throughput sequencing method.Nucleic acids research · 2010Article
- High-throughput multiplexed transcript analysis yields enhanced resolution of 5-hydroxytryptamine 2C receptor mRNA editing profiles.Molecular pharmacology · 2010Article
- Affect-related behaviors in mice misexpressing the RNA editing enzyme ADAR2.Physiology & behavior · 2009Article
- An innovative real-time PCR method to measure changes in RNA editing of the serotonin 2C receptor (5-HT(2C)R) in brain.Journal of neuroscience methods · 2009Article
- RNA editing of the serotonin 2C receptor and expression of Galpha(q) protein: genetic mouse models do not support a role for regulation or compensation.Journal of neurochemistry · 2009Article
- A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accumulating evidence suggests that altered RNA editing of the serotonin 2C receptor (HTR2C) is involved in the pathophysiology of mental disorders and the action of antidepressants. Estimating RNA editing of HTR2C in various samples is a first step to understanding its pathophysiological roles. Here, we developed a high-throughput quantification method of RNA editing efficiency by pyrosequencing. By optimizing the dispensation order, the RNA editing efficiency of all five RNA editing sites including consecutively ordered sites in HTR2C was obtained. More importantly, our method made it possible to determine the content of partial HTR2C isoforms, which enabled us to monitor possible functional changes of HTR2C. This method was validated in both oligonucleotide and RT-PCR product templates, and showed good correlation with conventional cloning-sequencing analysis. Our method could be a valuable tool in the rapid assessment of RNA editing status, including assessment of natural variations, alterations in disease tissues, and responses to drugs.
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