ArticleJournal of bacteriology2005
Genetic analysis identifies a function for the queC (ybaX) gene product at an initial step in the queuosine biosynthetic pathway in Escherichia coli.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Structural and functional insights into QueC-family protein in QatABCD anti-phage system.Nature communications · 2026Article
- Deazaguanylation is a nucleobase-protein conjugation required for type IV CBASS immunity.Science (New York, N.Y.) · 2025Article
- Queuine Salvaging in the Human ParasiteCells · 2022Article
- Impact of queuosine modification of endogenousFrontiers in molecular biosciences · 2022Article
- Epoxyqueuosine Reductase QueH in the Biosynthetic Pathway to tRNA Queuosine Is a Unique Metalloenzyme.Biochemistry · 2021Article
- Synthesis ofBeilstein journal of organic chemistry · 2021Article
- Insights into the Periplasmic Proteins ofInternational journal of molecular sciences · 2019Article
- Superior cellular activities of azido- over amino-functionalized ligands for engineered preQRNA biology · 2018Article
- Identification of a Novel Epoxyqueuosine Reductase Family by Comparative Genomics.ACS chemical biology · 2017Article
- Antimicrobial peptides trigger a division block in Escherichia coli through stimulation of a signalling system.Nature communications · 2016Article
- The zinc-responsive regulon of Neisseria meningitidis comprises 17 genes under control of a Zur element.Journal of bacteriology · 2012Article
- YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors.RNA (New York, N.Y.) · 2010Article
- A new member of the Escherichia coli fad regulon: transcriptional regulation of fadM (ybaW).Journal of bacteriology · 2009Article
- The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps.Biochemistry · 2009Article
- Escherichia coli QueD is a 6-carboxy-5,6,7,8-tetrahydropterin synthase.Biochemistry · 2009Article
- Biosynthesis of 7-deazaguanosine-modified tRNA nucleosides: a new role for GTP cyclohydrolase I.Journal of bacteriology · 2008Article
- Deciphering deazapurine biosynthesis: pathway for pyrrolopyrimidine nucleosides toyocamycin and sangivamycin.Chemistry & biology · 2008Article
- Environmental and growth phase regulation of the Streptococcus gordonii arginine deiminase genes.Applied and environmental microbiology · 2008Article
- Confirmation of a second natural preQ1 aptamer class in Streptococcaceae bacteria.RNA (New York, N.Y.) · 2008Article
- Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies.Journal of molecular biology · 2007Article
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2 authors.
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Abstract
Queuosine (Q), one of the most complex modifications occurring at the wobble position of tRNAs with GUN anticodons, is implicated in a number of biological activities, including accuracy of decoding, virulence, and cellular differentiation. Despite these important implications, its biosynthetic pathway has remained unresolved. Earlier, we observed that a naturally occurring strain of Escherichia coli B105 lacked Q modification in the tRNAs. In the present study, we developed a genetic screen to map the defect in E. coli B105 to a single gene, queC (renamed from ybaX), predicted to code for a 231-amino-acid-long protein with a pI of 5.6. As analyzed by mobility of tRNA(Tyr) on acid urea gels and two-dimensional thin-layer chromatography of the modified nucleosides, expression of QueC from a plasmid-borne copy confers a Q+ phenotype to E. coli B105. Further, analyses of tRNA(Tyr) from E. coli JE10651 (queA mutant), its derivative generated by deletion of chromosomal queC (queA deltaqueC), and E. coli JE7325, deficient in converting preQ0 to preQ1, have provided the first genetic evidence for the involvement of QueC at a step leading to production of preQ0, the first known intermediate in the generally accepted pathway that utilizes GTP as the starting molecule. In addition, we discuss the possibilities of collaboration of QueC with other cellular proteins in the production of preQ0.
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