ArticleProtein science : a publication of the Protein Society2005
Expression of human peripheral cannabinoid receptor for structural studies.
Article in Protein science : a publication of the Protein Society, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 90 citations in OpenAlex.
- Engineered Chimera Protein Constructs to Facilitate the Production of Heterologous Transmembrane Proteins inInternational journal of molecular sciences · 2024Review
- Special issue for Klaus Gawrisch.Biophysical journal · 2023Article
- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Poly-Histidine-Tagged Protein Purification Using Immobilized Metal Affinity Chromatography (IMAC).Methods in molecular biology (Clifton, N.J.) · 2023Article
- Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Optimizing the Expression of Human Dopamine Receptors inInternational journal of molecular sciences · 2021Article
- Site-selective labeling and electron paramagnetic resonance studies of human cannabinoid receptor CBBiochimica et biophysica acta. Biomembranes · 2021Article
- Cholesterol as a modulator of cannabinoid receptor CBScientific reports · 2021Article
- Efficient production of a functional G protein-coupled receptor in E. coli for structural studies.Journal of biomolecular NMR · 2021Article
- Recombinant Expression and Purification of Cannabinoid Receptor CBMethods in molecular biology (Clifton, N.J.) · 2021Article
- Thermostability of a recombinant G protein-coupled receptor expressed at high level in mammalian cell culture.Scientific reports · 2020Article
- Expression and purification of recombinant G protein-coupled receptors: A review.Protein expression and purification · 2020Review
- Probing thermostability of detergent-solubilized CBThe Journal of biological chemistry · 2020Article
- Expression and Preparation of a G-Protein-Coupled Cannabinoid Receptor CBCurrent protocols in protein science · 2019Article
- Expression, Purification and Characterization of the Human Cannabinoid 1 Receptor.Scientific reports · 2018Article
- Expression and purification of the mammalian translocator protein for structural studies.PloS one · 2018Article
- Application of Strep-Tactin XT for affinity purification of Twin-Strep-tagged CBProtein expression and purification · 2017Article
- Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II.Methods in enzymology · 2017Article
- Detergent optimized membrane protein reconstitution in liposomes for solid state NMR.Biochemistry · 2014Article
- Global fold of human cannabinoid type 2 receptor probed by solid-state 13C-, 15N-MAS NMR and molecular dynamics simulations.Proteins · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Human peripheral-type cannabinoid receptor (CB2) was expressed in Escherichia coli as a fusion with the maltose-binding protein, thioredoxin, and a deca-histidine tag. Functional activity and structural integrity of the receptor in bacterial protoplast membranes was confirmed by extensive binding studies with a variety of natural and synthetic cannabinoid ligands. E. coli membranes expressing CB2 also activated cognate G-proteins in an in vitro coupled assay. Detergent-solubilized receptor was purified to 80%-90% homogeneity by affinity chromatography followed by ion-exchange chromatography. By high-resolution NMR on the receptor in DPC micelles, it was determined that purified CB2 forms 1:1 complexes with the ligands CP-55,940 and anandamide. The receptor was successfully reconstituted into phosphatidylcholine bilayers and the membranes were deposited into a porous substrate as tubular lipid bilayers for structural studies by NMR and scattering techniques.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.