ArticleAmerican journal of respiratory cell and molecular biology2006
Identification of glycosaminoglycans in human airway secretions.
Article in American journal of respiratory cell and molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 71 citations in OpenAlex.
- Hyaluronan and layilin mediate loss of airway epithelial barrier function induced by cigarette smoke by decreasing E-cadherin.The Journal of biological chemistry · 2012Trial
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- Optimization of chemical transfection in airway epithelial cell lines.BMC biotechnology · 2025Article
- A possible important regulatory role of estrogen in obstructive sleep apnea hypoventilation syndrome.Frontiers in medicine · 2025Review
- Extracellular matrix-derived materials for tissue engineering and regenerative medicine: A journey from isolation to characterization and application.Bioactive materials · 2024Review
- Viral Genetic Determinants of Prolonged Respiratory Syncytial Virus Infection Among Infants in a Healthy Term Birth Cohort.The Journal of infectious diseases · 2023Article
- Mucus increases cell iron uptake to impact the release of pro-inflammatory mediators after particle exposure.Scientific reports · 2023Article
- Enterovirus D68 Infection in Human Primary Airway and Brain Organoids: No Additional Role for Heparan Sulfate Binding for Neurotropism.Microbiology spectrum · 2022Article
- Membrane-bound mucins of the airway mucosal surfaces are densely decorated with keratan sulfate: revisiting their role in the Lung's innate defense.Glycobiology · 2021Article
- Article
- Extracellular matrix remodelling in COPD.European respiratory review : an official journal of the European Respiratory Society · 2020Review
- The role of hyaluronic acid and hyaluronidase-1 in obstructive sleep apnoea.Scientific reports · 2020Article
- Article
- Hyaluronic Acid in the Third Millennium.Polymers · 2018Review
- ManLMN is a glucose transporter and central metabolic regulator in Streptococcus pneumoniae.Molecular microbiology · 2016Article
- Hyaluronan Rafts on Airway Epithelial Cells.The Journal of biological chemistry · 2016Article
- Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells.Mediators of inflammation · 2016Article
- Respiratory Syncytial Virus Attachment Glycoprotein Contribution to Infection Depends on the Specific Fusion Protein.Journal of virology · 2016Article
- Functional Analysis of the 60-Nucleotide Duplication in the Respiratory Syncytial Virus Buenos Aires Strain Attachment Glycoprotein.Journal of virology · 2015Article
- Inhibition of Pasteurella multocida Adhesion to Rabbit Respiratory Epithelium Using Lectins.Veterinary medicine international · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Glycosaminoglycans (GAGs), known to be present in airway mucus, are macromolecules with a variety of structural and biological functions. In the present work, we used fluorophore-assisted carbohydrate electrophoresis (FACE) to identify and relatively quantify GAGs in human tracheal aspirates (HTA) obtained from healthy volunteers. Primary cultures of normal human bronchial epithelial (NHBE) and submucosal gland (SMG) cells were used to assess their differential contribution to GAGs in mucus. Distribution was further assessed by immunofluorescence in human trachea tissue sections and in cell cultures. HTA samples contained keratan sulfate (KS), chondroitin/dermatan sulfate (CS/DS), and hyaluronan (HA), whereas heparan sulfate (HS) was not detected. SMG cultures secreted CS/DS and HA, CS/DS being the most abundant GAGs in these cultures. NHBE cells synthesized KS, HA, and CS/DS. Confocal microscopy showed that KS was exclusively found at the apical border of NHBE cells and on the apical surface of ciliated epithelial cells in tracheal tissues. CS/DS and HA were present in both NHBE and SMG cells. HS was only found in the extracellular matrix in trachea tissue sections. In summary, HTA samples contain KS, CS/DS, and HA, mirroring a mixture of secretions originated in surface epithelial cells and SMGs. We conclude that surface epithelium is responsible for most HA and all KS present in secretions, whereas glands secrete most of CS/DS. These data suggest that, in diseases where the contribution to secretions of glands versus epithelial cells is altered, the relative concentration of individual GAGs, and therefore their biological activities, will also be affected.
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Registered trials
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