ArticleProceedings of the National Academy of Sciences of the United States of America2005
Interfacial metal and antibody recognition.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- New insights into antibody structure with implications for specificity, variable region restriction and isotype choice.Nature reviews. Immunology · 2025Review
- Potent and durable control of mesothelin-expressing tumors by a novel T cell-secreted bi-specific engager.Journal for immunotherapy of cancer · 2025Article
- Editorial: Mechanisms and strategies of unconventional antibody diversification for greater immune adaptability.Frontiers in immunology · 2023Article
- Antigen binding by conformational selection in near-germline antibodies.The Journal of biological chemistry · 2022Article
- Heterotypic interactions drive antibody synergy against a malaria vaccine candidate.Nature communications · 2022Article
- Controlled hierarchical self-assembly of networked coordination nanocapsulesChemical science · 2020Article
- Ion Binding Properties of a Naturally Occurring Metalloantibody.Antibodies (Basel, Switzerland) · 2020Article
- Breaking the law: unconventional strategies for antibody diversification.Nature reviews. Immunology · 2019Review
- The Therapeutic Antibody LM609 Selectively Inhibits Ligand Binding to Human αStructure (London, England : 1993) · 2017Article
- Article
- Prevalence and gene characteristics of antibodies with cofactor-induced HIV-1 specificity.The Journal of biological chemistry · 2015Article
- Hedgehog pathway antagonist 5E1 binds hedgehog at the pseudo-active site.The Journal of biological chemistry · 2010Article
- Metal-directed protein self-assembly.Accounts of chemical research · 2010Review
- The crystal structure of sphingosine-1-phosphate in complex with a Fab fragment reveals metal bridging of an antibody and its antigen.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Inhibition of HIV-1 entry by antibodies: potential viral and cellular targets.Journal of internal medicine · 2007Review
- Prediction of the functional class of metal-binding proteins from sequence derived physicochemical properties by support vector machine approach.BMC bioinformatics · 2006Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
The unique ligation properties of metal ions are widely exploited by proteins, with approximately one-third of all proteins estimated to be metalloproteins. Although antibodies use various mechanisms for recognition, to our knowledge, none has ever been characterized that uses an interfacial metal. We previously described a family of CD4-reactive antibodies, the archetype being Q425. CD4:Q425 engagement does not interfere with CD4:HIV-1 gp120 envelope glycoprotein binding, but it blocks subsequent steps required for viral entry. Here, we use surface-plasmon resonance to show that Q425 requires calcium for recognition of CD4. Specifically, Q425 binding of calcium resulted in a 55,000-fold enhancement in affinity for CD4. X-ray crystallographic analyses of Q425 in the presence of Ca(2+), Ba(2+), or EDTA revealed an exposed metal-binding site, partially coordinated by five atoms contributed from four antibody complementarity-determining regions. The results suggest that Q425 recognition of CD4 involves direct ligation of antigen by the Q425-held calcium, with calcium binding each ligating atom of CD4 with approximately 1.5 kcal/mol of binding energy. This energetic contribution, which is greater than that from a typical protein atom, demonstrates how interfacial metal ligation can play a unique role in antigen recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.